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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 24, 2020 |
| Title |
Flavivirus induces and antagonizes antiviral RNA interference in both mammals and mosquitoes |
| Organisms |
Aedes aegypti; Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Mosquito-borne flaviviruses maintain life cycles in mammals and mosquitoes. RNA interference (RNAi) has been demonstrated as an anti-flavivirus mechanism in mosquitoes; however, whether and how flavivirus induces and antagonizes RNAi-mediated antiviral immunity in mammals remains unknown. Here we showed that NS2A of Dengue virus-2 (DENV2) act as a viral suppressor of RNAi (VSR). When NS2A-mediated RNAi suppression was disabled, the resulting mutant DENV2 induced Dicer-dependent production of abundant DENV2-derived siRNAs in differentiated mammalian cells. Importantly, VSR-disabled DENV2 showed severe replication defects in mosquito and mammalian cells, and mice, which were rescued by the deficiency of RNAi. Moreover, NS2As of multiple flaviviruses act as VSRs in vitro and during viral infection in both organisms. Overall, our findings demonstrate that antiviral RNAi can be induced by flavivirus, while flavivirus uses NS2A as bona fide VSR to evade RNAi in mammals and mosquitoes, highlighting the importance of RNAi in flaviviral vector-host life cycles.
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| Overall design |
Small RNA profile of virus-infected cells.
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| Contributor(s) |
Qiu Y, Xu YP, Wang M, Miao M, Zhou H, Xu J, Kong J, Zhang D, Li RT, Zhang RR, Guo Y, Li XF, Cui J, Qin CF, Zhou X |
| Citation(s) |
32076641 |
| Submission date |
Jun 25, 2019 |
| Last update date |
Feb 24, 2020 |
| Contact name |
xi zhou |
| E-mail(s) |
zhouxi@wh.iov.cn
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| Organization name |
Wuhan University
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| Department |
State Key Laboratory of Virology, College of Life Sciences
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| Street address |
Luojia Hill
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430072 |
| Country |
China |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL21020 |
Illumina HiSeq 2000 (Aedes aegypti) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA550985 |
| SRA |
SRP212063 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133284_RAW.tar |
400.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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