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| Status |
Public on Jun 29, 2022 |
| Title |
Genome-wide maps of chromatin state in iBMDM (immortalized bone-marrow-derived macrophages) cells without or with virus infection. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
iBMDM cells were infected with VSV (Vesicular Stomatitis Virus) or HSV-1 (Herpes Simplex Virus 1) for 8 hours, followed by CHIP-seq analysis with H3K27AC antibody (Abcam, ab4729). We find that chromatin state changed with virus infection, some enhacer and superenhancer were induced to promote anti-viral gene expression.
Conclusions: virus infection induce enhancer and superenhancer activation.
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| Overall design |
iBMDM cells were infected with VSV or HSV-1 for 8 hours, then cells were fixed and lysed, chromatin DNA were immunoprecipitated with H3K27AC antibody, followed by CHIP-seq analysis. Input was a pool of total DNA from uninfected iBMDM cells.
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| Contributor(s) |
Cao L, You F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 03, 2019 |
| Last update date |
Jun 29, 2022 |
| Contact name |
Shengde Liu |
| E-mail(s) |
liusd1311@163.com
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| Organization name |
Peking University Health Science Center
|
| Street address |
38 Xueyuan Rd , Haidian District
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| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA552532 |
| SRA |
SRP212897 |
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