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| Status |
Public on Mar 02, 2020 |
| Title |
Expression data of mouse alveolar macrophage cell (MH-S) colonies stimulated with LPS |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Under microscope, MH-S cells show a heterogenous population. By clonal dilution, we generated single cell colonies and found that a set of clones that secreted higher the IL-4Ra regulating protein (“High IL-4Ra activity clones”) and set of colonies secreted lower amount (“Low IL-4Ra activity clones”).
We performed microarrays to detail the global gene expression analysis comparing high IL-4Ra activity and low IL-4Ra activity MH-S clones stimulated with or without LPS. At this time point, roughly 50 genes annotated as “extracellular” were significantly upregulated more than two-fold in LPS stimulated clones compared to unstimulated ones. Of these, only seven genes showed higher expression in the high IL-4Ra activity clones compared to low IL-4Ra activity clones.
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| Overall design |
Three high IL-4Ra activity and three low IL-4Ra activity MH-S clones stimulated with or without LPS for 16 hrs were selected for RNA extraction and hybridization on Affymetrix microarrays. Total 12 samples were analysed.
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| Contributor(s) |
Wermeling F, Panda SK |
| Citation(s) |
31980518 |
| Submission date |
Jul 09, 2019 |
| Last update date |
Mar 02, 2020 |
| Contact name |
Fredrik Wermeling |
| E-mail(s) |
fredrik.wermeling@ki.se
|
| Organization name |
Karolinska Institute, Stockholm, Sweden
|
| Department |
Division of Rheumatology, Department of Medicine, Solna
|
| Lab |
Fredrik Wermeling´s lab
|
| Street address |
Visionsgatan 18, Center for molecular medicine, Karolinska University Hospital
|
| City |
Sockholm |
| State/province |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (12)
|
| GSM3934417 |
MH-S clone 7_low IL-4Ra activity_unstimulation |
| GSM3934418 |
MH-S clone 29_ low IL-4Ra activity_ unstimulation |
| GSM3934419 |
MH-S clone 75_ low IL-4Ra activity_ unstimulation |
| GSM3934420 |
MH-S clone 69_ high IL-4Ra activity_ unstimulation |
| GSM3934421 |
MH-S clone 73_ high IL-4Ra activity_ unstimulation |
| GSM3934422 |
MH-S clone 83_ high IL-4Ra activity_ unstimulation |
| GSM3934423 |
MH-S clone 7_ low IL-4Ra activity_ 16h LPS stimulation |
| GSM3934424 |
MH-S clone 29_ low IL-4Ra activity_ 16h LPS stimulation |
| GSM3934425 |
MH-S clone 75_ low IL-4Ra activity_ 16h LPS stimulation |
| GSM3934426 |
MH-S clone 69_ high IL-4Ra activity_ 16h LPS stimulation |
| GSM3934427 |
MH-S clone 73_ high IL-4Ra activity_ 16h LPS stimulation |
| GSM3934428 |
MH-S clone 83_ high IL-4Ra activity_ 16h LPS stimulation |
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| Relations |
| BioProject |
PRJNA553553 |
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