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| Status |
Public on Jan 13, 2020 |
| Title |
The Dynamic Transcriptional Cell Atlas of Testis Development During Human Puberty |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The human testis undergoes dramatic developmental and structural changes during puberty, including proliferation and maturation of niche/somatic cells, and the onset of spermatogenesis. Here, we profiled and analyzed single-cell transcriptomes of ~10,000 testicular cells from four boys spanning puberty, and compared to infants and adults. During puberty, undifferentiated spermatogonia first expand and then differentiate, prior to gametogenesis. Notably, we identify a common pre-pubertal progenitor for Leydig and myoid cells, and reveal candidate factors/pathways for pubertal differentiation. Furthermore, pre-pubertal Sertoli cells form two states that differ in mitochondrial/metabolic transcription, which converge to a single mature population during puberty. Roles for testosterone in Sertoli cell maturation, antimicrobial peptide secretion and spermatogonial differentiation are revealed through analyses of testosterone-suppressed transgender female testis and via in vitro seminiferous tubule culturing. Overall, our transcriptional atlas of the developing human testis provides major insights into developmental changes and key factors/pathways that accompany male puberty.
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| |
|
| Overall design |
Single-cell RNA-seq of testicular cells from different ages of boys in puberty. Testosterone-suppressed transgender female testis samples are included.
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| |
|
| Contributor(s) |
Guo J, Cairns B |
| Citation(s) |
31928944 |
| Submission date |
Jul 11, 2019 |
| Last update date |
Feb 01, 2020 |
| Contact name |
Jingtao Guo |
| E-mail(s) |
jingtao.guo@hci.utah.edu
|
| Phone |
801-585-1823
|
| Organization name |
University of Utah School of Medicine
|
| Department |
Department of Surgery Division of Urology
|
| Lab |
Jingtao Guo
|
| Street address |
2000 Circle of Hope
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (11)
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|
| Relations |
| BioProject |
PRJNA554092 |
| SRA |
SRP214255 |
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