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| Status |
Public on Jan 31, 2020 |
| Title |
Deep sequencing identified serum extracellular vesicle delivered miR-181a-5p as an indicator for bone-metastatic prostate cancer (microarray) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Purpose: The goals of this study are to compare the serum extracellular vesicle (EV) delivered miRNA levels of patients with bone-metastatic prostate cancer (PCa), non-bone -metastatic PCa and benign prostatic hyperplasia (BPH), and to identify EV-delivered microRNAs in patient’s serum as indicators for bone-metastatic PCa.
Methods:Serum extracellular vesicle delivered miRNA profiles of patients with bone-metastatic PCa or non-bone -metastatic PCa or BPH were generated by miRNA chip array, using Agilent-070156 Human_miRNA_V21.0_Microarray plateform.
Results: Differential analysis showed the expressions of 27 EV delivered miRNAs were significantly different between serum of patients with bone-metastatic PCa and non-bone-metastatic PCa with a p value <0.05. the expressions of 5 EV delivered miRNAs were confirmed with qRT–PCR.
Conclusions: Serum EV-delivered miR-181a-5p is a promising diagnostic biomarker for bone-metastatic PCa.
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| Overall design |
Serum extracellular vesicle delivered miRNA profiles of patients with bone-metastatic PCa or non-bone -metastatic PCa or benign prostatic hyperplasia were generated by miRNA chip array, and contrastive analysis.
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| |
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| Contributor(s) |
Fang Y, Wang Y |
| Citation(s) |
33391510 |
| Submission date |
Jul 15, 2019 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Yanqing Wang |
| E-mail(s) |
iwangyq@163.com
|
| Organization name |
Shanghai Renji Hospital
|
| Department |
Urology
|
| Street address |
NO. 160 Pujian Road
|
| City |
Shanghai |
| ZIP/Postal code |
200127 |
| Country |
China |
| |
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| Platforms (1) |
| GPL25134 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version) |
|
| Samples (38)
|
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| Relations |
| BioProject |
PRJNA554594 |
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