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| Status |
Public on Nov 19, 2019 |
| Title |
Coagulation FXIII-A protein expression defines three novel sub-populations in pediatric B-cell progenitor acute lymphoblastic leukemia characterized by distinct gene expression signatures |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Leukemic B-cell precursor (BCP) lymphoblasts were recently identified as a novel expression site for coagulation factor XIII subunit A (FXIII-A). The FXIII-A negative subgroup was significantly associated with the ‘B-other’ genetic category and had an unfavorable disease outcome
RNA was extracted from bone marrow lymphoblasts of 42 pediatric patients with BCP-acute lymphoblastic leukemia (ALL). FXIII-A expression was determined by multiparameter FC. Genetic diagnosis was based on conventional cytogenetic method and fluorescence in situ hybridization. Affymetrix GeneChip Human Primeview array was used to analyze global expression pattern of 28869 well-annotated genes.
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| Overall design |
Sub-groups were createdtwo way: 1) based on the protein expression of FXIII-A: FXIII-A negative group (<20% FXIII-A positive lymphoblasts), FXIII-A dim group (20%-79% FXIII-A positive lymphoblasts), FXIII-A positive group (≥80% FXIII-A positive lymphoblasts) 2) based on genetic sub-type: non-B-other: - low-risk including t(12;21)/ETV6/RUNX1(TEL-AML1) and high hyperdiploidy (51-65 chromosome), - high-risk including MLL translocations, iAMP21, complex karyotype, near haploidy (chromosome number 23-29), and low hypodiploidy (chromosome<45) - intermediate risk with t(1;19) translocations, B-other: anything else not belonging to the upper categories which are currently genetically unclassified
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| Contributor(s) |
Gyurina K, Kárai B, Ujfalusi A, Hevessy Z, Barna G, Jáksó P, Pálfi-Mészáros G, Póliska S, Scholtz B, Kappelmayer J, Zahuczky G, Kiss C |
| Citation(s) |
31709175 |
| Submission date |
Jul 18, 2019 |
| Last update date |
Nov 23, 2019 |
| Contact name |
Szilard Poliska |
| E-mail(s) |
poliska@dote.hu
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| Phone |
+3652416432
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| Organization name |
University of Debrecen
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| Department |
Biochemistry and Molecular Biology
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| Lab |
Nuclear Hormone Receptor Research Laboratory
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| Street address |
Egyetem ter 1.
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| City |
Debrecen |
| ZIP/Postal code |
H-4012 |
| Country |
Hungary |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA555255 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE134480_RAW.tar |
94.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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