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| Status |
Public on Dec 27, 2019 |
| Title |
Super-enhancer-associated oncogenes as novel therapeutic targets in osteosarcoma (Microarray gene expression data) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
CDK7 is a component of the general transcription factor IIH, which regulates RNAPII initiation and elongation.THZ2, a new molecular inhibitor, can completely inhibit the phosphorylation of the established intracellular CDK7 substrate RNAPII CTD at Ser-2, -5 and -7 through irreversible covalent binding to CDK7.
Gene expression profiling was then performed to investigate the THZ2-induced transcription effect, and search the subset of sensitive genes in these 2 osteosarcoma cell lines.
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| Overall design |
Total RNA was extracted from U2-OS or SJSA-1 cells treated with DMSO (control), 25, 100, and 400 nM THZ2 for 6 hours, respectively, using TRIzol reagent.An Affymetrix GeneChip® PrimeView™ Human Gene Expression Array was used for the microarray analysis. The hybridization data were analyzed using Affymetrix GeneChip Command Console Software.
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| Contributor(s) |
Zhang J, Liu W, Zou C, Zhao Z, Lai Y, Shi Z, Xie X, Huang G, Wang Y, Zhang X, Fan Z, Su Q, Yin J, Shen J |
| Citation(s) |
31937612 |
| Submission date |
Jul 22, 2019 |
| Last update date |
Mar 29, 2020 |
| Contact name |
Jiajun Zhang |
| E-mail(s) |
zhangjiajun92@126.com
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| Organization name |
the First Affiliated Hospital of Sun Yat-Sen University
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| Street address |
#58，Zhongshan 2nd Road
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| City |
Guangzhou |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE134605 |
Super-enhancer-associated oncogenes as novel therapeutic targets in osteosarcoma |
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| Relations |
| BioProject |
PRJNA555842 |
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