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| Status |
Public on Apr 20, 2020 |
| Title |
Modeling hypoxia induced neuropathies using a fast and scalable human motor neuron differentiation system |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Human pluripotent stem cells carry great potential to provide novel insights into disease mechanisms by enabling generation and study of functional cell types under cell culture conditions, including human motor neurons (MNs). MN disease are a group of conditions where these nerves progressively loose function. Recent findings using animal model systems suggest a previously unappreciated role for mRNA mis-localization in disease development and progression. However, model systems to investigate this novel disease link in the human context are currently absent. Here we describe a scalable suspension-based differentiation system to generate large numbers of hPSCs derived neuronal progenitors that can further differentiate efficiently into functional stem cell derived human motor neurons (sMN) within only 3 weeks. To be able to investigate potential differences in mRNA transcripts between sMN cell soma and neurites we further established a membrane-based culture system that allows efficient fractionation of cell soma and neurites.
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| |
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| Overall design |
Quantification of transcript abundance in soma and neurites of hypoxia and control human motor neurons
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| |
|
| Contributor(s) |
Holger R, Taliaferro JM |
| Citation(s) |
32386561 |
| Submission date |
Jul 23, 2019 |
| Last update date |
Jul 20, 2020 |
| Contact name |
Matthew Taliaferro |
| Organization name |
University of Colorado Anschutz Medical Campus
|
| Department |
Biology
|
| Street address |
12801 E 17th Ave, RC1 South Room 10403D
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (34)
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| Relations |
| BioProject |
PRJNA556250 |
| SRA |
SRP216073 |
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