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| Status |
Public on Jun 08, 2020 |
| Title |
The Evolving Immune Microenvironment of B-cell Acute Lymphoblastic Leukemia [human peripheral blood dataset] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single-cell RNA-seq analysis of primary human peripheral blood mononuclear cells (PBMCs) from two independent B-ALL patients (Texas Children's Hospital) and healthy PBMC donor samples (STEMCELL Technologies).
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| Overall design |
Single-cell RNA-sequencing libraries of primary human peripheral blood immune populations. B-ALL patient bone marrow samples were separated into CD19+ B cell and CD19-CD45+ non-B cell fractions by flow-based sorting and then mixed at a ratio of 1:5 CD19+:CD19-CD45+ followed by scRNA-Seq. Total CD45+ peripheral blood cells were sequenced from healthy donors.
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| Contributor(s) |
Witkowski MT, Dolgalev I, Tsirigos A, Aifantis I |
| Citation(s) |
32470390 |
| Submission date |
Jul 23, 2019 |
| Last update date |
Jun 08, 2020 |
| Contact name |
Igor Dolgalev |
| Organization name |
NYU Grossman School of Medicine
|
| Street address |
550 1st Ave
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (1) |
| GSM3967066 |
HEALTHY-PB1, HEALTHY-PB2, BALL-PB1, BALL-PB2 |
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| This SubSeries is part of SuperSeries: |
| GSE134759 |
The Evolving Immune Microenvironment of Human B-cell Acute Lymphoblastic Leukemia |
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| Relations |
| BioProject |
PRJNA556277 |
| SRA |
SRP216168 |
