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| Status |
Public on Apr 07, 2020 |
| Title |
Cryptic introns in lncRNAs recruit conserved Pir2/ARS2 protein to promote gene repression [ChIP-seq] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Long non-coding RNAs (lncRNAs) dynamically regulate gene expression during development and in response to environmental conditions. In S. pombe, lncRNAs repress the expression of nearby genes via a mechanism involving silencing effector proteins. In this study, we find that invasion of a lncRNA into the neighboring gene results in inclusion of a cryptic intron that is required for its repressive activity. We show that cryptic introns are targeted by the conserved protein Pir2/ARS2 in association with splicing factors, which provide a scaffold for the recruitment of RNA degradation factors and chromatin modifying activities.
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| |
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| Overall design |
ChIP-seq of Pir2-GFP in Schizosaccharomyces pombe.
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| Contributor(s) |
Thillainadesan G, Xiao H, Holla S, Dhakshnamoorthy J, Grewal SI |
| Citation(s) |
32415063 |
| Submission date |
Jul 31, 2019 |
| Last update date |
Jun 08, 2020 |
| Contact name |
Shiv Grewal |
| Phone |
2407607553
|
| Organization name |
NCI
|
| Department |
LBMB
|
| Lab |
Shiv Grewal
|
| Street address |
NCI bldg 37 Rm 6068 9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20584 |
Illumina NextSeq 500 (Schizosaccharomyces pombe) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE135161 |
Cryptic introns in lncRNAs recruit conserved Pir2/ARS2 protein to promote gene repression |
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| Relations |
| BioProject |
PRJNA557601 |
| SRA |
SRP216891 |
