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Series GSE136031 Query DataSets for GSE136031
Status Public on Aug 20, 2019
Title Expression data from 4T1 subclones derived from mammary fat pad tumors (MFP), axillary lymph node tumors (AxLN), and axillary lymph node-derived lung metastases (AxLN-LuM)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Expression data from 4T1 subclones derived from mammary fat pad tumors (MFP), axillary lymph node tumors (AxLN), and axillary lymph node-derived lung metastases (AxLN-LuM). In parallel, expression data, in the same subclones, of tail vein-derived (TV) lung metastases.
The mechanism of how lymph node metastases seed distant metastases is unknown. We used the 4T1 breast cancer cell line, which is an immune competent model of triple negative breast cancer and spontaneously metastasizes in balb/c mice.
4T1-GFP/fLuc cells were injected into MFP to form tumors and 4T1-mCherry/rLuc cells were injected into axillary lymph nodes to form tumors and then allowed to metastasize to lung. TV cells were allowed to metastasize in the lung. Cells were harvested at different time intervals after the injection. Tumors were extracted, dissociated, and then expanded in vitro to obtain MFP, AxLN, AxLN-LuM and TV-LuM subclones isolated after different time lags with respect to the injection.
 
Overall design Subclones were grown to 60-80% confluence for RNA extraction and hybridization on Affymetrix microarrays. Then, differentially expressed genes were assessed amongst the aforementioned subclones.
 
Contributor(s) Porrello A, Pecot CV
Citation(s) 31519896
Submission date Aug 19, 2019
Last update date Sep 23, 2019
Contact name Alessandro Porrello
E-mail(s) alessandro.porrello@gmail.com
Organization name University of North Carolina at Chapel Hill
Department Lineberger Comprehensive Cancer Center
Street address 450 West Drive
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platforms (1)
GPL17400 [MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version]
Samples (48)
GSM4039629 4T1-GFP/fLuc, murine breast cancer cells, bio rep1
GSM4039630 4T1-GFP/fLuc, murine breast cancer cells, bio rep2
GSM4039631 4T1-mCherry-rLuc, murine breast cancer cells, bio rep1
Relations
BioProject PRJNA560974

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Supplementary file Size Download File type/resource
GSE136031_RAW.tar 213.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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