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| Status |
Public on Dec 14, 2020 |
| Title |
RNA-seq analysis of mouse cardiomyocytes treated with dnMKL1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to analyze the impact of dnMKL1 on transcription during cardiomyocyte maturation in mouse
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| Overall design |
Wildtype mice were injected with AAV9-dnMKL1-GFP or AAV9-GFP specifically in cardiomyocytes at P1. Five biological replicates were analyzed in each group.
In each experiment, cardiomyocytes were isolated by retrograde collagenase perfusion and purified by fluorescence activated cell sorting.
Total RNA was extracted from FACS sorted cells. RNA-seq libraries were generated using SMART-seq and Nextera kits and were sequenced on a NextSeq550 sequencer. FASTQ Data were aligned by STAR and count matrix generated by FeatureCounts.
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| Contributor(s) |
Guo Y, Pu W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 20, 2019 |
| Last update date |
Dec 14, 2020 |
| Contact name |
Yuxuan Guo |
| E-mail(s) |
yuxuan.guo@childrens.harvard.edu
|
| Organization name |
Boston childrens hospital
|
| Department |
Department of Cardiology
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| Lab |
William Pu
|
| Street address |
320 Longwood Ave, Enders 1250,
|
| City |
boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE136096 |
Sarcomeric regulation of serum response factor signaling in cardiomyocyte maturation by actinin-2 |
|
| Relations |
| BioProject |
PRJNA561181 |
| SRA |
SRP218916 |
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