Expression data from "sensitized" mice exposed via respiratory tract to chemical (methylene diphenyldiisocyanate)-glutathione conjugates +/- chloride channel inhibitor (crofelemer)
Methylene diphenyl diisocyanate is a chemical known to cause asthma. The present study uses mice to investigate exposure-induced changes in lung gene expression and effects of a chloride channel inhibitor We used microarrays to detail global whole lung gene expression following respiratory tract exposure to methylene diphenyl diisocyanate (MDI) vs. control exposure in mice immune-sensitized to MDI by prior skin exposure. In some studies mice were given a chloride channel inhibitor (crofelemer) via the respiratory tract before MDI.
Overall design
Mice were immune-sensitized to methylene diphenyl diisocyanate by repeated skin exposure. Immune sensitized mice were subsequently exposed to methylene diphenyl diisocyanate (reaction products with glutathione) intranasally under isoflurane and perfused (airway lavaged) lung tissue was obtained 48 hours after final exposure. Stuudies were done in BalbC, C57BL6, and B-cell deficient mice. In some studies mice were given a chloride channel inhibitor (crofelemer) via the respiratory tract before MDI.
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