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| Status |
Public on Sep 04, 2020 |
| Title |
Time-resolved RNA atlas of early human T cell activation (miRNA) |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
MicroRNAs are central regulators of the T cell function. We explored RNA expression profiles over the initial 24 h of human CD4+ T cell activation. We found high similarity in time-resolved miRNA expression courses comparing independent activations and different donors. The detected miRNA expression patterns could be grouped into six classes only, each with a defined time course. MiR-155-5p known for its role in T cell immunity showed the most prevalent expression changes, quantified with an hourly increase of about 60 molecules/cell. As demonstrated for miRNA-155-5p, the analysis of time-resolved miRNA and mRNA expression data allowed to increase the validation rate of predicted miRNA targets to close to 90 %. Combining our time-resolved expression analysis with an absolute quantification of miRNA expression changes, gives new insights into miRNA regulatory networks and indicates the functional dominance of specific miRNAs within the early T cell activation.
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| Overall design |
Early human T cell activation
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| Contributor(s) |
Diener C, Hart M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 29, 2019 |
| Last update date |
Sep 06, 2020 |
| Contact name |
Tim Kehl |
| E-mail(s) |
tkehl@bioinf.uni-sb.de
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| Phone |
+49-681-302-68613
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| Organization name |
Saarland University
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| Department |
Center for Bioinformatics
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| Street address |
Campus E2.1, P.O. box 151150
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| City |
Saarbrücken |
| ZIP/Postal code |
66041 |
| Country |
Germany |
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| Platforms (1) |
| GPL25134 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version) |
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| Samples (78)
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| GSM4053254 |
Donor 1, Timepoint 6, Replicate A |
| GSM4053255 |
Donor 1, Timepoint 8, Replicate A |
| GSM4053256 |
Donor 1, Timepoint 10, Replicate A |
| GSM4053257 |
Donor 1, Timepoint 12, Replicate A |
| GSM4053258 |
Donor 1, Timepoint 14, Replicate A |
| GSM4053259 |
Donor 1, Timepoint 16, Replicate A |
| GSM4053260 |
Donor 1, Timepoint 18, Replicate A |
| GSM4053261 |
Donor 1, Timepoint 20, Replicate A |
| GSM4053262 |
Donor 1, Timepoint 22, Replicate A |
| GSM4053263 |
Donor 1, Timepoint 24, Replicate A |
| GSM4053264 |
Donor 1, Timepoint 0, Replicate B |
| GSM4053265 |
Donor 1, Timepoint 2, Replicate B |
| GSM4053266 |
Donor 1, Timepoint 4, Replicate B |
| GSM4053267 |
Donor 1, Timepoint 6, Replicate B |
| GSM4053268 |
Donor 1, Timepoint 8, Replicate B |
| GSM4053269 |
Donor 1, Timepoint 10, Replicate B |
| GSM4053270 |
Donor 1, Timepoint 12, Replicate B |
| GSM4053271 |
Donor 1, Timepoint 14, Replicate B |
| GSM4053272 |
Donor 1, Timepoint 16, Replicate B |
| GSM4053273 |
Donor 1, Timepoint 18, Replicate B |
| GSM4053274 |
Donor 1, Timepoint 20, Replicate B |
| GSM4053275 |
Donor 1, Timepoint 22, Replicate B |
| GSM4053276 |
Donor 1, Timepoint 24, Replicate B |
| GSM4053277 |
Donor 1, Timepoint 0, Replicate C |
| GSM4053278 |
Donor 1, Timepoint 2, Replicate C |
| GSM4053279 |
Donor 1, Timepoint 4, Replicate C |
| GSM4053280 |
Donor 1, Timepoint 6, Replicate C |
| GSM4053281 |
Donor 1, Timepoint 8, Replicate C |
| GSM4053282 |
Donor 1, Timepoint 10, Replicate C |
| GSM4053283 |
Donor 1, Timepoint 12, Replicate C |
| GSM4053284 |
Donor 1, Timepoint 14, Replicate C |
| GSM4053285 |
Donor 1, Timepoint 16, Replicate C |
| GSM4053286 |
Donor 1, Timepoint 18, Replicate C |
| GSM4053287 |
Donor 1, Timepoint 20, Replicate C |
| GSM4053288 |
Donor 1, Timepoint 22, Replicate C |
| GSM4053289 |
Donor 1, Timepoint 24, Replicate C |
| GSM4053290 |
Donor 2, Timepoint 0, Replicate A |
| GSM4053291 |
Donor 2, Timepoint 2, Replicate A |
| GSM4053292 |
Donor 2, Timepoint 4, Replicate A |
| GSM4053293 |
Donor 2, Timepoint 6, Replicate A |
| GSM4053294 |
Donor 2, Timepoint 8, Replicate A |
| GSM4053295 |
Donor 2, Timepoint 10, Replicate A |
| GSM4053296 |
Donor 2, Timepoint 12, Replicate A |
| GSM4053297 |
Donor 2, Timepoint 14, Replicate A |
| GSM4053298 |
Donor 2, Timepoint 16, Replicate A |
| GSM4053299 |
Donor 2, Timepoint 18, Replicate A |
| GSM4053300 |
Donor 2, Timepoint 20, Replicate A |
| GSM4053301 |
Donor 2, Timepoint 22, Replicate A |
| GSM4053302 |
Donor 2, Timepoint 24, Replicate A |
| GSM4053303 |
Donor 2, Timepoint 0, Replicate B |
| GSM4053304 |
Donor 2, Timepoint 2, Replicate B |
| GSM4053305 |
Donor 2, Timepoint 4, Replicate B |
| GSM4053306 |
Donor 2, Timepoint 6, Replicate B |
| GSM4053307 |
Donor 2, Timepoint 8, Replicate B |
| GSM4053308 |
Donor 2, Timepoint 10, Replicate B |
| GSM4053309 |
Donor 2, Timepoint 12, Replicate B |
| GSM4053310 |
Donor 2, Timepoint 14, Replicate B |
| GSM4053311 |
Donor 2, Timepoint 16, Replicate B |
| GSM4053312 |
Donor 2, Timepoint 18, Replicate B |
| GSM4053313 |
Donor 2, Timepoint 20, Replicate B |
| GSM4053314 |
Donor 2, Timepoint 22, Replicate B |
| GSM4053315 |
Donor 2, Timepoint 24, Replicate B |
| GSM4053316 |
Donor 2, Timepoint 0, Replicate C |
| GSM4053317 |
Donor 2, Timepoint 2, Replicate C |
| GSM4053318 |
Donor 2, Timepoint 4, Replicate C |
| GSM4053319 |
Donor 2, Timepoint 6, Replicate C |
| GSM4053320 |
Donor 2, Timepoint 8, Replicate C |
| GSM4053321 |
Donor 2, Timepoint 10, Replicate C |
| GSM4053322 |
Donor 2, Timepoint 12, Replicate C |
| GSM4053323 |
Donor 2, Timepoint 14, Replicate C |
| GSM4053324 |
Donor 2, Timepoint 16, Replicate C |
| GSM4053325 |
Donor 2, Timepoint 18, Replicate C |
| GSM4053326 |
Donor 2, Timepoint 20, Replicate C |
| GSM4053327 |
Donor 2, Timepoint 22, Replicate C |
| GSM4053328 |
Donor 2, Timepoint 24, Replicate C |
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| Relations |
| BioProject |
PRJNA562892 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE136626_RAW.tar |
546.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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