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| Status |
Public on Nov 02, 2019 |
| Title |
Transcriptional profiling reveals differential response to heat stress in Tharparker and Crossbred Cattle |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have used RNA-Seq to examine expression profiles of PBMCs from Crossbred and Thraparkar under heat Stress.
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| Overall design |
Tharparkar and Crossbred were subjected to heat for a period of 7 days. RNA was isolated from PBMCs of both genetic groups (n=5 for each genetic group), before ( 0 days ) and post heat stress (7 day ). The pooled RNA samples of each genetic group for both the individual time points were subjected to illumina sequencing .
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| Contributor(s) |
Gupta S, Singh P, Saxena A, Varshney A, Malla WA, Verma V, Sahu AR, Kumar P, Saxena S, Pandey A, Khan RI, Hosamani N, Praharaj MR, Kumar S, Singh G, Gandham RK, Tiwari AK, Sharma S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 29, 2019 |
| Last update date |
Nov 04, 2019 |
| Contact name |
Ravi Kumar Gandham |
| E-mail(s) |
gandham71@gmail.com
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| Phone |
+919458687850
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| Organization name |
NIAB
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| Street address |
Gowlidoddy
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| City |
Hyderabad |
| State/province |
Telangana |
| ZIP/Postal code |
500032 |
| Country |
India |
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| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
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| Samples (8)
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| GSM4054570 |
PBMCs of Crossbred cattle under heat stress at day 0, rep1 |
| GSM4054571 |
PBMCs of Crossbred cattle under heat stress at day 0, rep2 |
| GSM4054572 |
PBMCs of Crossbred cattle under heat stress at day 7, rep1 |
| GSM4054573 |
PBMCs of Crossbred cattle under heat stress at day 7, rep2 |
| GSM4054574 |
PBMCs of Tharparkar cattle under heat stress at day 0, rep1 |
| GSM4054575 |
PBMCs of Tharparkar cattle under heat stress at day 0, rep2 |
| GSM4054576 |
PBMCs of Tharparkar cattle under heat stress at day 7, rep1 |
| GSM4054577 |
PBMCs of Tharparkar cattle under heat stress at day 7, rep2 |
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| Relations |
| BioProject |
PRJNA562925 |
| SRA |
SRP219848 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE136652_RAW.tar |
3.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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