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| Status |
Public on Nov 25, 2008 |
| Title |
Genome wide analysis of gene expression of rat ES cells, rat embryonic fibroblast cells and mouse ES cells |
| Organisms |
Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Rat ES cells were derived using 3I medium from E4.5 blastocysts. Rat embryonic fibroblast cells were derived form E14.5 embryos. To analyze the mechanism under the selfrenewal of rat ES cells, microarrays were used for the genome wide analysis of gene expressoin profiles in rat ES cells. Rat embryonic fibroblast cells and mouse ES cells were tested at same time as control. Our results from clustering analysis demonstrated that the gene expression profile of rat ES cells resembles mouse ES cells, but not REFs.
Keyword: 3I medium; rat embryonic stem cells; mouse ES cells; rat embryonic fibroblast cells
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| Overall design |
Rat ES cells were cultured in 3I medium; rat embryonic fibroblast cells were derived and cultured GMEM/10% FBS; mouse ES cells (C57/BL6)were cultured in GMEM/10% FBS added LIF and feeder cells were removed before RNA extraction. Three replicates each.
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| |
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| Contributor(s) |
Ying Q, Li P |
| Citation(s) |
19109898 |
| Submission date |
Nov 20, 2008 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Qilong Ying |
| E-mail(s) |
qying@usc.edu
|
| Organization name |
USC
|
| Department |
Broad Center for Regenerative Medicine and Stem Cell Research
|
| Street address |
1501 San Pablo St, ZNI 507
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (2) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
|
| GSM344336 |
rat embryonic fibroblast cells sample1 |
| GSM344337 |
rat embryonic fibroblast cells sample2 |
| GSM344338 |
rat embryonic fibroblast cells sample3 |
| GSM344751 |
mouse embryonic stem cells sample1 |
| GSM344752 |
mouse embryonic stem cells sample2 |
| GSM344753 |
mouse embryonic stem cells sample3 |
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| Relations |
| BioProject |
PRJNA110431 |
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