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| Status |
Public on Oct 21, 2021 |
| Title |
Maternal protein restriction changes skeletal muscle metabolism and structural gene expression in aging rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We intended to evaluate the phenotypic characteristics of the muscle microenvironment, involving muscle fiber and extracellular matrix, in skeletal muscle of elderly rats, at 540 days, arising from maternal protein restriction.
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| Overall design |
Maternal protein restriction affects the postnatal skeletal muscle physiology with effects lasting until senility. Considering the different muscle adaptations after maternal protein restriction observed in previous studies, transcriptomic analysis has proven to be an excellent strategy to understand the impacts of fetal programming on skeletal muscle.
To investigate the morphological, metabolic, and molecular characteristics of skeletal muscle in aged rats subjected to maternal protein restriction, we used aged male rats (540 days old) born from dams fed with protein restricted diet (6% protein) during
gestation and lactation. Using molecular and morphological techniques, we evaluated the Soleus (SOL) and Extensor Digitorum Longus (EDL) muscles. Muscle fiber crosssectional area (CSA) was measured and gene expression of oxidative (Succinate Dehydrogenase-Sdha and Citrate Synthase-CS) and glycolytic (Lactate Dehydrogenase-Ldha) markers were evaluated by RT-qPCR. Global transcriptome analysis also was performed to identify deregulated genes, following the RT-qPCR validation. The oxidative SOL muscle displayed decrease in muscle fiber CSA and in the expression of oxidative metabolism marker, up-regulation of anabolic Igf-1, structural Chad, and Fmod gene, and down-regulated Hspb7 gene. The glycolytic EDL muscle had no changes in muscle fiber CSA and in expression of oxidative metabolism genes, while the gene expression of Chad was up-regulated and Myog
gene was down-regulated. Collectively, our data show that maternal protein restriction induced changes in the expression of metabolic, anabolic, myogenic and structural genes, mainly in oxidative SOL muscle, in aged offspring rats.
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| Contributor(s) |
Valente JS, Perez ÉS, Zanella BT, Paula TG, Santos SA, Duran BO, Carvalho RF, Justulin LA, Fantinatti BE, Silva MD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 09, 2019 |
| Last update date |
Oct 21, 2021 |
| Contact name |
Bruno Evaristo de Almeida Fantinatti |
| E-mail(s) |
bfantinatti@gmail.com
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| Phone |
+5514981322558
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| Organization name |
Unesp
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| Department |
Morphology
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| Street address |
Prof Antonio Celso Wagner Zanin
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| City |
Botucatu |
| State/province |
São Paulo |
| ZIP/Postal code |
18618-689 |
| Country |
Brazil |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (12)
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| GSM4066834 |
Sample 12: maternal protein restriction RNAseq |
| GSM4066835 |
Sample 13: maternal protein restriction RNAseq |
| GSM4066836 |
Sample 15: maternal protein restriction RNAseq |
| GSM4066837 |
Sample 23: control RNAseq |
| GSM4066838 |
Sample 28: control RNAseq |
| GSM4066839 |
Sample 29: control RNAseq |
| GSM4066840 |
Sample 32: maternal protein restriction RNAseq |
| GSM4066841 |
Sample 33: maternal protein restriction RNAseq |
| GSM4066842 |
Sample 35: maternal protein restriction RNAseq |
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| Relations |
| BioProject |
PRJNA564541 |
| SRA |
SRP221029 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE137094_normalized.counts.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
| GSE137094_raw.counts.txt.gz |
433.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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