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| Status |
Public on Oct 01, 2023 |
| Title |
Transcriptomic profiling of electrophysiologically characterized human iPSC-derived motor neurons having SOD1 A4V mutation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To find the molecular basis for abnormal excitability in human iPSC-derived motor neurons with the SOD1 A4V mutation and its involvement in risk of cell loss, we conducted a patch-seq, which combines genome-wide RNA sequencing and patch-clamp recording. This experiment enables excitability and gene expression to be measured simultaneously at a single cell level such that the links between the two could be explored.
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| Overall design |
MNs differentiated from SOD1 A4V/+ iPSC lines and their isogenic controls were purified by introducing a GFP reporter under the control of the Hb9 promoter and GFP flow sorting. Three weeks after differentiation on P0-1 mouse cortical glial cells, whole cell current clamp recordings were made followed by single MN isolation for single cell Smart-seq2. Smart-seq2 analysis was performed on 192 neurons in two independent batches.
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| Contributor(s) |
Lee S, Wiskow O, Ghosh S, Bean BP, Eggan K, Woolf CJ |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 NS105076 |
Unravelling mechanisms and novel therapeutic targets for peripheral neuropathy and neuropathic pain |
CHILDREN'S HOSPITAL |
CLIFFORD J WOOLF |
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| Submission date |
Sep 27, 2019 |
| Last update date |
Oct 01, 2023 |
| Contact name |
Seungkyu Lee |
| E-mail(s) |
sklee0125@gmail.com
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| Phone |
6176201238
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| Organization name |
Boston Children's Hospital
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| Department |
Neurobiology Dept.
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| Lab |
Clifford Woolf lab
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| Street address |
3 Blackfan circle, CLS bldg. #12260
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (192)
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| Relations |
| BioProject |
PRJNA575003 |
| SRA |
SRP223741 |
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