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| Status |
Public on Oct 03, 2019 |
| Title |
Expression array of mouse bone marrow-derived macrophages and osteoclasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In response to the cytokines, macrophage colony-stimulating factor and receptor activator of NF-kB ligand, monocyte precursors differentiate into bone marrow-derived macrophages (BMDMs) that ultimately fuse to form multi-nucleated osteoclasts, following a tightly controlled genetic program where specific sets of genes are differentially expressed.
We used microarrays to examine the gene expression profile underlying mouse osteoclast differentiation.
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| Overall design |
Bone marrow-derived macrophages and osteoclasts were lysed at specified stages of differentiation for RNA extraction and hybridization to Affymetrix microarrays for a time-dependent profile of the BMDM-osteoclast transition. We selected BMDM-osteoclasts at five time-points: before differentiation, i.e. BMDMs (day 0), during differentiation (day 1, 2 and 3) and the mature osteoclasts (day 5).
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| Contributor(s) |
Zhu L, Weiss S |
| Citation(s) |
32024800 |
| Submission date |
Oct 02, 2019 |
| Last update date |
Nov 09, 2020 |
| Contact name |
Lingxin Zhu |
| Organization name |
Univeristy of Michigan in Ann Arbor
|
| Street address |
210 Washtenaw Ave, Life Sciences Institute, Room 5008
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
|
| Samples (5)
|
| GSM4105465 |
wild-type BMDM to osteoclast differentiation at day 0 |
| GSM4105466 |
wild-type BMDM to osteoclast differentiation at day 1 |
| GSM4105467 |
wild-type BMDM to osteoclast differentiation at day 2 |
| GSM4105468 |
wild-type BMDM to osteoclast differentiation at day 3 |
| GSM4105469 |
wild-type BMDM to osteoclast differentiation at day 5 |
|
| Relations |
| BioProject |
PRJNA575514 |
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