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| Status |
Public on Feb 27, 2020 |
| Title |
smallRNA-sequencing reveals microRNAs as endogenous Toll-like receptor ligands causing neuroinflammation |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
To identify microRNAs as TLR-activating molecules we have induced apoptosis in primary cortical neurons to trigger specific microRNA release into media. smallRNA sequencing revealed potential microRNAs ligands derived from dying neurons and their corresponding media that harbor already described TLR7/8 activating sequences. In line with this finding, about 80% of our differentially present miRNAs were able to activate TLR7/8 reporter cell lines. Furthermore, microRNAs miR-340-3p and miR-132-5p induced a specific cytokine and chemokine release form microglia leading to neuronal death. Within our systematic approach, we have identified TLR paralog-specific activating microRNAs with the potential to serve as biomarkers for brain diseases.
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| |
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| Overall design |
Apoptotic neurons release microRNAs as endogenous signaling molecules extracellular that are endosmal TLR7 and TLR8 ligands.
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| |
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| Contributor(s) |
Lehnardt S, Wallach T, Staszewski O |
| Citation(s) |
31940779 |
| Submission date |
Oct 07, 2019 |
| Last update date |
Feb 27, 2020 |
| Contact name |
Ori Staszewski |
| E-mail(s) |
ori.staszewski@uniklinik-freiburg.de
|
| Organization name |
University Medical Center Freiburg
|
| Department |
Institute of Neuropathology
|
| Street address |
Breisacher Str. 64
|
| City |
Freiburg |
| ZIP/Postal code |
D-79106 |
| Country |
Germany |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA576261 |
| SRA |
SRP224658 |
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