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| Status |
Public on Dec 20, 2019 |
| Title |
Transcriptome changes in bovine oocytes caused by microinjection |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: In this study, we investigated the effect of microinjection on oocytes transcriptome changes through scrambled siRNA injection.
Method: Scrambled siRNA injection followed by RNA-seq of single oocytes.
Result:Our result showed 119 transcripts were differentially expressed of which 76 were down-regulated after scrambled siRNA injection.
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| Overall design |
Conculsion: Biology process of ATP synthesis, molecular transportation and regulation of protein polyubiquitination can act as reference of background effect when conduct siRNA injection experiment with oocytes.
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| Contributor(s) |
Tan M, van Tol HT, Mokry M, Stout TA, Roelen BA |
| Citation(s) |
32641751 |
| Submission date |
Nov 04, 2019 |
| Last update date |
Jul 13, 2020 |
| Contact name |
Bernard A.J. Roelen |
| E-mail(s) |
B.A.J.Roelen@uu.nl
|
| Organization name |
Utrecht University
|
| Department |
Department of Farm Animal Health
|
| Lab |
Animal Reproduction
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| State/province |
The Netherlands |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
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| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA587409 |
| SRA |
SRP228532 |
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