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| Status |
Public on Mar 05, 2020 |
| Title |
Raptor is required for β-cell metabolic coupling and repressing an α cell program before onset of hyperglycemia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Raptor deficient mice showed diabetic phenotype, to dissect the effect of hyperglycemia, we isolated euglycemic 2-week-old β cells to perform microarray.
To determine the influence of Raptor deficiency on β cells independent of hyperglycemia, we hace employed whole transcriptome expression profiling as a discovery platform to identify differentially expressed genes compared with wild type controls.
We isolated mice islets, then disgested them into single cells by 0.25% EDTA, and sorted for GFP by FACS.
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| Overall design |
Raptor regulated gene expression in β cells from knockout mice was measured at 2 weeks after birth. Age-matched wild type mice were set as controls.
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| Contributor(s) |
Wang Q, Ning G |
| Citation(s) |
32439909 |
| Submission date |
Nov 12, 2019 |
| Last update date |
Jun 01, 2020 |
| Contact name |
Qinglei Yin |
| E-mail(s) |
yinqinglei128@163.com
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| Organization name |
Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine
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| Street address |
Ruijin Second Road No.197
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| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA588976 |
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