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| Status |
Public on Feb 10, 2021 |
| Title |
Expression data of the livers in mouse with iso-α-acids intake |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Scope: Alcoholic liver disease (ALD) is a major cause of chronic liver disease and is induced by alcohol consumption. Acetaldehyde produced by alcohol metabolism enhances the fibrosis of the liver through hepatic stellate cells. Additionally, alcohol administration causes the accumulation of reactive oxygen species (ROS), which induce hepatocyte-injury-mediated lipid peroxidation. The purpose of this study was to investigate the protective effects of iso-α-acids against alcoholic liver injury in hepatocytes in mice.
Methods and results: C57BL/6N mice were fed diets containing isomerized hop extract, which mainly consists of iso-α-acids. After 7 days of feeding, acetaldehyde was administered by a single intraperitoneal injection. The acetaldehyde-induced increases in serum AST and ALT levels were suppressed by iso-α-acids intake. Hepatic gene expression analyses showed the upregulation of the glutathione-S-transferase, alcohol dehydrogenase and aldehyde dehydrogenase genes. In vitro, iso-α-acids induced the nuclear translocation of nuclear factor erythroid 2-like 2 (Nfe2l2; Nrf2), a master regulator of antioxidant and detoxifying systems, and upregulated the enzymatic activities of glutathione-S-transferase and aldehyde dehydrogenase. Conclusions: These results suggest that iso-α-acids intake prevents alcoholic liver disease injury by reducing oxidative stress via the Nrf2-mediated pathway.
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| Overall design |
Eight-week-old male C57BL/6NCrSlc mice were purchased from Japan SLC (Shizuoka, Japan) and were housed in controlled temperature (24±1°C), humidity (50-70%), and light (12 h light-dark cycle) conditions. They were fed the AIN-93G diet (Oriental Yeast, Tokyo, Japan) for a week and then were divided into two groups based on similar average body weight. The control diet group (n = 10) was fed the AIN-93G diet, and the iso-α-acids diet group (n = 10) was fed the AIN-93G diet containing iso-α-acids (0.5%, w/w). Mice in the control group were pair-fed the control diet to the ad libitum intake of the iso-α-acid group for a week. After a week feeding period, animals were treated with an intraperitoneal (i.p.) injection of acetaldehyde (200 mg/kg). Tail vein blood samples were collected at 1, 2, and 5 h after the acetaldehyde injection. Liver samples were collected after blood sampling under deep anesthesia. The protocols for the animal experiments were approved by the Animal Use Committee of Sapporo Holdings Ltd., Research and Development Division. DNA microarray analysis was performed on liver samples from a total of ten mice (five mice each from the iso-α-acid and control diet groups) by choosing average individuals from each group on the basis of their serum enzyme values.
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| Contributor(s) |
Takase T, Toyoda T, Kobayashi N, Inoue T, Ishijima T, Tsuchiya Y, Okada S |
| Citation(s) |
33544749 |
| Submission date |
Nov 14, 2019 |
| Last update date |
Feb 10, 2021 |
| Contact name |
Shinji Okada |
| E-mail(s) |
asoka@mail.ecc.u-tokyo.ac.jp
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| Organization name |
The University of Tokyo
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| Street address |
1-1-1 Yayoi
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| City |
Bunkyo-ku, Tokyo |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA589569 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE140387_RAW.tar |
10.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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