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| Status |
Public on Nov 21, 2019 |
| Title |
Transcriptomic analysis of adhesive capsulitis of the shoulder |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We compared genes from tissue of patients with adhesive capsulitis (AC) with those having surgery for shoulder instability to determine potential biomarkers specific to AC through transcriptomic analysis. Our results presented increased expression of PDGFB, COL18A1 and MMP9 in patients with AC, while TNFA expression was reduced.
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| Overall design |
Tissue samples were collected from the anterior capsule and rotator interval during surgery. Total RNA was extracted and RNA-sequencing-based transcriptomics were performed. Bioinformatic analyses were carried out to identify biological processes and pathways enriched in this dataset. A number of genes deemed differentially expressed in RNA-sequencing analysis were validated using real time RT-PCR.
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| Contributor(s) |
Kamal N, McGee SL, Brown G, Beattie S, Eng K, Collier F, Gill S, Page R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 20, 2019 |
| Last update date |
Nov 27, 2019 |
| Contact name |
Nima Kamal |
| E-mail(s) |
nimakamal94@gmail.com
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| Organization name |
Deakin University
|
| Department |
School of Medicine
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| Lab |
Molecular and Medical Research
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| Street address |
75 Pigdons Rd
|
| City |
Waurn Ponds |
| State/province |
VIC |
| ZIP/Postal code |
3216 |
| Country |
Australia |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA590709 |
| SRA |
SRP230798 |
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