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| Status |
Public on Dec 03, 2019 |
| Title |
HSV-1 infection of neural system-derived cells, including SH-SY5Y and U87MG cells at 0, 4 and 10 hour post infection with an MOI of 0.01,to identify the differentially expressed miRNAs and perform pathway enrichment analysis using KEGG. |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
we employed human miRNA Microarray assay to identify differentially expressed (DE) miRNAs in response to HSV-1 infection of SH-SY5Y and U87MG cells. The significantly DE miRNAs existed in U87MG but not SH-SY5Y cells at 4 hours post-infection (hpi), HSV-1 latency-associated transcripts (LAT)-derived miRNAs were not abundantly expressed in both SH-SY5Y and U87MG cells, meanwhile, HSV-1 replication was significantly inhibited in U87MG but not SH-SY5Y. Pathway enrichment analysis results showed that target genes of 10 down-regulated DE miRNAs were significantly enriched in Janus kinase-signal transducers and activator of transcription (JAK-STAT) signaling and Herpes simplex virus infection in U87MG cells at 4 hpi. These results indicated that DE of miRNAs may contribute to the HSV-1 replication inhibition in U87MG cells and also may be linked to HSV-1 latency in neurons.
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| Overall design |
The celluar miRNAs expression profile respond to HSV-1 infection in SH-SY5Y and U87MG cells at 0, 4 and 10 hours post infection with an MOI of 0.01. 1 independent experiment was performed at the each time point (total 6 experiments)
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| Contributor(s) |
duan y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 02, 2019 |
| Last update date |
Dec 06, 2019 |
| Contact name |
yongzhong duan |
| E-mail(s) |
duanyongzhong@imbcams.com.cn
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| Phone |
+8613888329604
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| Organization name |
kunming medical university
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| Street address |
chongrong west road, chenggong new district
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| City |
kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
650500 |
| Country |
China |
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| Platforms (1) |
| GPL25134 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA592912 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE141241_RAW.tar |
36.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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