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| Status |
Public on Dec 10, 2019 |
| Title |
Capsular glycan recognition provides antibody-mediated protection against tuberculosis |
| Organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
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| Summary |
Increasing evidence suggests that antibodies (Abs) can have protective roles in M. tuberculosis (Mtb) infection but knowledge of the most relevant protective antigens and epitopes in humans is limited.
Using novel glycan arrays, we establish that human serum IgG induced against the M. tuberculosis (Mtb) capsular polysacharide arabinomannan (AM) in natural Mtb infection is highly heterogeneous in its binding specificity and differs in both its reactivity to oligosaccharide (OS) motifs within AM and its functions between BCG vaccination and/or controlled (latent) versus uncontrolled (TB) M. tuberculosis infection. We show that anti-AM IgG from asymptomatic but not diseased individuals is protective, and provide data suggesting a role of IgG2 and specific AM oligosaccharides. Filling a gap in the current knowledge of protective antigens in humans, our human data support the key role of the M. tuberculosis surface glycan AM and suggest the importance of targeting specific glycan epitopes within AM in antibody-mediated immunity against TB.
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| Overall design |
Sera from 10 asymptomatic TST+/IGRA+ subjects and 13 symptomatic TB subjects were analyzed for anti-AM OS IgG reactivity. The anti-AM OS reactivities were then evaluated for correlations with Ab-mediated protective functions against M. tuberculosis.
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| Contributor(s) |
Chen T, Blanc C, Joe M, Lowary TL, Achkar JM |
| Citation(s) |
31935198 |
| Submission date |
Dec 09, 2019 |
| Last update date |
Mar 10, 2020 |
| Contact name |
Jacqueline Achkar |
| E-mail(s) |
jacqueline.achkar@einsteinmed.org
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| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park Avenue
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| City |
Bronx |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL27885 |
LAM Subarray 30 - Antigens 10/22/2015 |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA594355 |
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