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| Status |
Public on Dec 26, 2019 |
| Title |
RNA-seq in liver cancer cell and cinobufagin treatment cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cinobufagin, belonged to a kind of cardiotonic steroids (CSs), derived from the extracts of toad venom, which presented the significant anticancer properties as inhibitors of Na+/K+-ATPase (NKA) in many cancer cells. Nowadays, cinobufagin was usually used to apply for clinical treatments of patients in advanced stage of cancer, and improved their quality of life and prolonged their survival period. Unfortunately, long-term drug treatment had also led to multi-drug-resistance (MDR) likely other chemotherapy drugs. However, this detailed mechanism was not still clear. In the present study, we noticed that cinobufagin could trigger the protective autophagy to suppress cells apoptosis in liver cancer HepG2 and Huh-7 cells by inhibition of PI3K-AKT-mTOR pathway using RNA-seq method. We also confirmed that cinobufagin could inhibit cells proliferation and induce cells apoptosis, and generated cells autophagy by up-regulation expression of LC3B-II, Beclin 1 and Atg12-atg5. More importantly, autophagy inhibitor (MRT68921) enhances the anti-proliferation and pro-apoptosis effects of cinobufagin in HCC cells. Therefore, we thought that a combination of cinobufagin and autophagy inhibitors may be a potential effective strategy in treatment of HCC.
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| Overall design |
Examination of cinobufagin treatment for 0, 12, and 24 h in 2 cell types.
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| Contributor(s) |
Xu Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 25, 2019 |
| Last update date |
Dec 28, 2019 |
| Contact name |
Zhongwei Xu |
| E-mail(s) |
xzw113@gmail.com
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| Phone |
02284876563
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| Organization name |
Logistics University of Chinese People’s Armed Police Force
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| Street address |
No.1 Huizhi Huan Road , DongLi district, Tianjin City, China, Shuguang Zhou
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| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300309 |
| Country |
China |
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| Platforms (1) |
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| Samples (16)
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| GSM4232495 |
Huh-7 treated with cinobufagin for 12 h-1 |
| GSM4232496 |
Huh-7 treated with cinobufagin for 12 h-2 |
| GSM4232497 |
Huh-7 treated with cinobufagin for 12 h-3 |
| GSM4232498 |
Huh-7 treated with cinobufagin for 24 h-1 |
| GSM4232499 |
Huh-7 treated with cinobufagin for 24 h-2 |
| GSM4232500 |
Huh-7 treated with cinobufagin for 24 h-3 |
| GSM4232501 |
HepG2 control 1 |
| GSM4232502 |
HepG2 treated with cinobufagin for 12 h-1 |
| GSM4232503 |
HepG2 treated with cinobufagin for 12 h-2 |
| GSM4232504 |
HepG2 treated with cinobufagin for 12 h-3 |
| GSM4232505 |
HepG2 treated with cinobufagin for 24 h-1 |
| GSM4232506 |
HepG2 treated with cinobufagin for 24 h-2 |
| GSM4232507 |
HepG2 treated with cinobufagin for 24 h-3 |
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| Relations |
| BioProject |
PRJNA597601 |
| SRA |
SRP238746 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142588_RAW.tar |
4.2 Mb |
(http)(custom) |
TAR (of XLS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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