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Series GSE142595 Query DataSets for GSE142595
Status Public on Dec 24, 2021
Title scRNA-seq of peripheral blood from West Nile Virus-infected patients
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We profile the peripheral blood of patients infected with West Nile Virus with divergent disease-trajectories (West Nile Encephalitis, West Nile Fever, and asymptomatic) during relatively acute infection and at a convalescent timepoint (~90-days later) using single-cell RNA sequencing in an effort to uncover determinants of disease progression and flesh out the landscape of infection. In the blood of the infected patients, stratified cell-states involved in acute viral infection resolve into more homogenous states at the follow-up blood draws, A dramatic shared transcriptional shift between the primary blood-draws during acute infection and the 90-day follow-ups in all observed compartments allows us to highlight multiple cell-type and cell-state-specific patterns of gene expression.
 
Overall design Single-cell RNA on patient samples infected with West Nile Virus
 
Contributor(s) Fikrig E, Fleming I, Halfer D, Kleinstein SH, Love CJ, Montgomery RR, Murray KO, Ronca S, Shalek A, Wang X
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Submission date Dec 26, 2019
Last update date Dec 25, 2021
Contact name Ira Daniel Klein Fleming
E-mail(s) ifleming@mit.edu
Organization name MIT
Street address 400 Main St
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (54)
GSM4232596 172169_071717_PBMC_scRNA-seq_2017_Thawed_1
GSM4232597 172169_071717_PBMC_scRNA-seq_2017_Thawed_2
GSM4232598 172169_111617_PBMC_scRNA-seq_2017_Thawed_1
Relations
BioProject PRJNA597718
SRA SRP238793

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142595_RAW.tar 136.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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