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| Status |
Public on Dec 09, 2020 |
| Title |
Metabolism in synovial macrophages are reprogrammed by synovial fibroblasts under inflammatory condition |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophages have plasticity to adapt microenvironment. In joint tissue, synovial macrophages (SM) and synovial fibroblasts (SF) are maintained in the homeostasis. In Rheumatoid arthritis, crosstalk between SM and SF via inflammatory response induce abnormal activation in respective cells and contribute to disease progression. However, the activation mechanisms in SM which are encouraged by SF are largely unclear. Here, we demonstrated metabolic reprogramming and immunological activation in SM by secretary stimulations from SF using primary culture synovial cell derived from arthritis model mice. To analyze interaction between SM and SF, primary culture of murine synovial cells was performed, respectively. RNA-seq analysis showed SF express abundant secretion-related gene. Thus, we investigated whether conditioned medium from SF (SF-CM) affects biological activity in SM. As the results, SF-CM condition induced both glycolysis and mitochondrial respiration to SM with increased uptake of glucose and glutamine at least, accompanied with cell survival. In addition, several inflammation markers were also upregulated in SM by SF-CM condition. Taken together, these results suggest that metabolic reprogramming were induced in SM by secretory stimulations from SF, followed by activated inflammatory response and long-live. These indicate that such phenotypes of SM may contribute to chronic inflammation in Rheumatoid arthritis
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| Overall design |
mRNA profiles of primary synovial macrophages and synovial fibroblasts obtained from swollen ankle in CAIA mice were generated by deep sequencing, in triplicate, using Illumina Miseq.
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| Contributor(s) |
Imai Y, Saeki N |
| Citation(s) |
33256735 |
| Submission date |
Dec 26, 2019 |
| Last update date |
Dec 09, 2020 |
| Contact name |
Yuuki Imai |
| E-mail(s) |
y-imai@m.ehime-u.ac.jp
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| Phone |
+81-89-960-5925
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| Organization name |
Ehime University
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| Department |
Proteo-Science Center
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| Lab |
Division of Integrative Pathophysiology
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| Street address |
Shitsukawa
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| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA597752 |
| SRA |
SRP238820 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142607_FPKM_SM_SF.xlsx |
2.9 Mb |
(ftp)(http) |
XLSX |
| GSE142607_Processed_data_modified.xlsx |
7.3 Mb |
(ftp)(http) |
XLSX |
| GSE142607_tmm_SM_SF.xlsx |
6.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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