|
| Status |
Public on Jan 09, 2020 |
| Title |
Histone Exchange is Associated with Activator Function at Transcribed Promoters and with Repression at Histone Loci |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Transcription in eukaryotes correlates with major chromatin changes, including the replacement of old nucleosomal histones by new histones at the promoters of genes. The role of these histone exchange events in transcription remains unclear. In particular, the causal relationship between histone exchange and activator binding, preinitiation complex (PIC) assembly, and/or subsequent transcription remains unclear. Here, we provide evidence that histone exchange at gene promoters is not simply a consequence of PIC assembly or transcription but instead is mediated by activators. We further show that not all activators up-regulate gene expression by inducing histone turnover. Thus, histone exchange does not simply correlate with transcriptional activity, but instead reflects the mode of action of the activator. Last, we show that histone turnover is not only associated with activator function but also plays a role in transcriptional repression at the histone loci.
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| |
|
| Overall design |
ChIP-seq of newly incorporated HA-tagged H3 and total H3 in G1-phase synchronized cells upon depletion of TBP
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| |
|
| Contributor(s) |
Kassem S, Ferrari P, Hughes AL, Soudet J, Rando OJ, Strubin M |
| Citation(s) |
32917590 |
| Submission date |
Jan 08, 2020 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Julien Soudet |
| E-mail(s) |
julien.soudet@unige.ch
|
| Organization name |
Université de Genève
|
| Department |
BICEL
|
| Street address |
30 quai Ansermet
|
| City |
Geneva |
| ZIP/Postal code |
1204 |
| Country |
Switzerland |
| |
|
| Platforms (2) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
| GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
|
| Samples (14)
|
|
| Relations |
| BioProject |
PRJNA599949 |
| SRA |
SRP267463 |
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