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| Status |
Public on Apr 10, 2009 |
| Title |
MUC1-induced transcriptional alterations in rat 3Y1 embryonic fibroblasts |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
The MUC1 oncoprotein is aberrantly overexpressed in diverse human malignancies including breast and lung cancer. Although MUC1 modulates the activity of several transcription factors, there is no information regarding the effects of MUC1 on global gene expression patterns and the potential role of MUC1-induced genes in predicting outcome for cancer patients. We have developed an experimental model of MUC1-induced transformation that has identified the activation of gene families involved in oncogenesis, angiogenesis and extracellular matrix remodeling. A set of experimentally-derived MUC1-induced genes associated with tumorigenesis was applied to the analysis of breast and lung adenocarcinoma cancer databases. A 35-gene MUC1-induced tumorigenesis signature (MTS) predicts significant decreases in both disease-free and overall survival in patients with breast (n = 295) and lung (n = 442) cancers. The data demonstrate that the MUC1 oncoprotein contributes to the regulation of genes that are highly predictive of clinical outcome in breast and lung cancer patients.
Keywords: Expression profiling by microarray
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| Overall design |
To investigate the genes associated with MUC1-CD-induced transformation and tumorigenesis, we performed expression profiling of 3Y1/Vector (empty vector-transfected 3Y1) and 3Y1/MUC1-CD (MUC1-CD-transfected 3Y1) cells growing in vitro, and of 3Y1/MUC1-CD cells growing as tumor xenografts in athymic mice. Cells grown in vitro or as tumor xenografts were lysed to collect total RNA for hybridization with GeneChip® Rat Genome 230 2.0 Arrays.
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| Contributor(s) |
Pitroda SP, Khodarev NN, Beckett MA, MacDermed DM, Huang L, Kufe DW, Weichselbaum RR |
| Citation(s) |
19289846, 20459602 |
| Submission date |
Jan 08, 2009 |
| Last update date |
Nov 08, 2019 |
| Contact name |
Sean Pravin Pitroda |
| E-mail(s) |
spitroda@uchicago.edu
|
| Organization name |
The University of Chicago
|
| Department |
Radiation and Cellular Oncology
|
| Street address |
5841 South Maryland Avenue, MC 1105
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (6)
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| GSM358693 |
MUC1-CD-transfected 3Y1 cells grown in vitro, biological replicate 1 |
| GSM358694 |
MUC1-CD-transfected 3Y1 cells grown in vitro, biological replicate 2 |
| GSM358695 |
MUC1-CD-transfected 3Y1 cells grown in vivo, biological replicate 1 |
| GSM358696 |
MUC1-CD-transfected 3Y1 cells grown in vivo, biological replicate 2 |
| GSM358697 |
Empty vector-transfected 3Y1 cells grown in vitro, biological replicate 1 |
| GSM358698 |
Empty vector-transfected 3Y1 cells grown in vitro, biological replicate 2 |
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| Relations |
| BioProject |
PRJNA111519 |
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