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| Status |
Public on May 28, 2004 |
| Title |
Microarray Based Comparison of two Amplification Methods For Nanogram Amounts of Total RNA |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Two T7 based methods One round of Amplification (Affymetrix) and Two round of Amplification were compared to two Ribo-SPIA based systems, RiboSPIA and pico Ribo SPIA systems. Data for One Round of amplification , Two round of amplification and RiboSPIA are listed here.
All Hybridisation were performed using Affymetrix Mouse 430-2 gene chips. Data were all scaled to 500 and scaling factor average was 3.6 +/- 0.9, background intensities 46 +/- 5 , noise 2.9 +/- 0.6, rawQ 1.5 +/- 0.2
Keywords: other
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| Contributor(s) |
Singh R, Maganti RJ, Jabba SV, Wangemann P |
| Citation(s) |
15613496 |
| Submission date |
May 26, 2004 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Philine Wangemann |
| E-mail(s) |
wange@vet.ksu.edu
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| Phone |
785-532-4863
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| Organization name |
Kansas State University
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| Department |
Anatomy and Physiology
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| Lab |
Cell Physiology Laboratory
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| Street address |
1600 Dension Avenue
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| City |
Manhattan |
| State/province |
KS |
| ZIP/Postal code |
66506 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA89973 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE1435_RAW.tar |
236.8 Mb |
(http)(custom) |
TAR (of CEL, RPT, TXT) |
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