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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 20, 2021 |
| Title |
Unsupervised cell interaction profiling based on multiplet RNA sequencing reveals major architectural differences between small intestinal and colonic epithelium. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cellular identity in complex multicellular organisms is strictly maintained over the course of life. This control is achieved in part by the organ structure itself, such that neighboring cells influence each other’s identity. However, large-scale investigation of the cellular interactome has been technically challenging. Here, we develop CIM-seq, an unsupervised and high-throughput method to analyze direct physical cell interactions between every cell type in a given tissue. CIM-seq is based on RNA sequencing of incompletely dissociated cells, followed by computational deconvolution of these into their constituent cell types using particle swarm optimization. We use CIM-seq to define the cell interaction landscape of the mouse small intestinal and colonic epithelium uncovering both known and novel interactions. Specifically, we find that the general architecture of the stem cell niche is radically different between the two tissues. In small intestine, the stem-paneth cell interaction forms an exceptionally strong and exclusive niche, in which paneth cells provide Wnt ligands1. In colonic epithelium, no similar compartment exists to support stem cells, and Wnt signaling is provided by a mesenchymal cell layer. However, colonic stem cells are associated with a subset of goblet cells expressing the wound healing marker Plet1, suggesting an additional level of structural control in the colon. These results identify novel cellular interactions specific for the colonic stem cell niche and shed light on a previously unappreciated complexity of the tissue organization and biology of the colon. CIM-seq is broadly applicable to studies that aim to simultaneously investigate the constituent cell types and the global interaction profile in a specific tissue.
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| Overall design |
Singlet and multiplet RNAseq data from C57BL/6J mouse colon, small intestine and spleen
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| Contributor(s) |
Serviss JT, Andrews N, Andersson AB, Dzwonkowska E, Heijboer R, Gerling M, Enge M, Geyer N |
| Citation(s) |
34253926 |
| Submission date |
Jan 14, 2020 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Martin Enge |
| E-mail(s) |
martin.enge@ki.se
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| Organization name |
Karolinska Institute
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| Department |
Dep of Oncology-Pathology
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| Street address |
CCK, Z4
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| City |
Stockholm |
| ZIP/Postal code |
S-171 76 |
| Country |
Sweden |
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| Platforms (1) |
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| Samples (7501)
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| Relations |
| BioProject |
PRJNA601201 |
| SRA |
SRP241947 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE143639_RAW.tar |
547.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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