|
| Status |
Public on May 05, 2020 |
| Title |
Chromatin remodeling in bovine embryos indicates species-specific regulation of genome activation |
| Organism |
Bos taurus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Characterization of genome-wide chromatin accessibility in bovine oocytes and preimplantation embryos using ATAC-seq
|
| |
|
| Overall design |
ATAC-seq libraries were prepared from bovine GV oocytes and in vitro produced preimplantation embryos (2-cell, 4-cell, 8-cell, and morula-stage embryos). For each developmental stage, ATAC-seq libraries were prepared for three biological replicates (cells derived from three separate oocyte collections). A subset of embryos from each collection were concurrently cultured in the presence of a transcriptional inhibitor (α-amanitin), to interrogate the causal relationship between embryonic transcription and chromatin remodeling.
|
| |
|
| Contributor(s) |
Halstead MM, Ross PJ |
| Citation(s) |
32943640 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HD070044 |
Epigenetic control of preimplantation embryo development |
UNIVERSITY OF CALIFORNIA DAVIS |
Pablo Juan Ross |
|
| Submission date |
Jan 14, 2020 |
| Last update date |
Oct 06, 2020 |
| Contact name |
Michelle M Halstead |
| E-mail(s) |
mhalstead226@gmail.com
|
| Organization name |
University of California, Davis
|
| Department |
Animal Science
|
| Lab |
Ross Lab
|
| Street address |
Meyer Hall, 450 Bioletti Way
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
|
| Samples (30)
|
|
| Relations |
| BioProject |
PRJNA601200 |
| SRA |
SRP241938 |
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