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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 01, 2020 |
| Title |
Estrogen receptor alpha signaling establishes a sexually dimorphic regulatory node of energy expenditure |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Estrogen receptor a (ERa) signaling in the ventromedial hypothalamus (VMH) contributes to energy homeostasis by modulating physical activity and thermogenesis. However, the precise neuronal populations involved remain undefined. Using single-cell RNA transcriptomics and in situ hybridization, we describe six neuronal populations in the mouse VMH. ERa is enriched in populations showing sex biased expression of reprimo (Rprm), tachykinin 1 (Tac1), and prodynorphin (Pdyn). Female biased expression of Tac1 and Rprm is patterned by ERa-dependent repression during male development, whereas male biased expression of Pdyn is maintained by circulating testicular hormone in adulthood. Chemogenetic activation of ERa+ VMH neurons stimulates heat generation and movement in both sexes. However, silencing Rprm gene function increases core temperature selectively in females and ectopic Rprm expression in males is associated with reduced core temperature. Together these findings reveal a role for Rprm in temperature regulation and ERa in the masculinization of neuron populations that underlie energy expenditure.
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| Overall design |
Single cell RNA sequencing of cells in the mouse ventromedial hypothalamus. N = 3 male and 3 female mice sacrificed on post-natal day 10, and FACS purified
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| Contributor(s) |
van Veen JE, Kammel L, Bunda P, Shum M, Reid M, Massa M, Arneson D, Park J, Zhang Z, Joseph A, Hrncir H, Liesa M, Arnold A, Yang X, Correa S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 16, 2020 |
| Last update date |
Apr 03, 2020 |
| Contact name |
John Edward van Veen |
| E-mail(s) |
jevanveen@gmail.com
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| Organization name |
UCLA
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| Department |
Integrative Biology and Physiology
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| Lab |
van Veen
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| Street address |
610 Charles E young Dr
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA601766 |
| SRA |
SRP242299 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE143818_RAW.tar |
2.2 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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