|
| Status |
Public on Jan 14, 2009 |
| Title |
TNF-a-induced MEK/ERK-dependent regulation of Cartilage Matrix Genes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
|
| Summary |
TNF-a is increased in the synovial fluid of patients with rheumatoid arthritis and osteoarthritis. TNF-a activates MEK/ERK in chondrocytes; however the overall functional relevance of MEK/ERK to TNF-a-regulated gene expression in chondrocytes is unknown. Chondrocytes were treated with TNF-a with or without the MEK1/2 inhibitor U0126 for 24 h. Microarray analysis was used to identify genes regulated by TNF-a in a MEK1/2-dependent fashion.
Keywords: compound, signalling response
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| |
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| Overall design |
Primary chondrocytes from the femoral condyles of neonatal rats were treated with DMSO (control sample), TNF-a, U0126 (selective MEK1/2 inhibitor) or TNF-a and U0126. Two biological replicates were collected for each treatment. Total RNA was collected and analyzed by Affymetrix Microarray.
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| |
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| Contributor(s) |
Rockel JS, Bernier SM, Leask A |
| Citation(s) |
19144181 |
| Submission date |
Jan 13, 2009 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Jason Scott Rockel |
| E-mail(s) |
jsrockel@uwo.ca
|
| Phone |
519-661-2111
|
| Organization name |
CIHR Group in Skeletal Development and Remodeling
|
| Department |
Anatomy and Cell Biology
|
| Lab |
Dr. Andrew Leask
|
| Street address |
The University of Western Ontario
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 5C1 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
|
| Samples (8)
|
| GSM359894 |
DMSO-treated chondrocytes, biological replicate 1 |
| GSM359895 |
DMSO-treated chondrocytes, biological replicate 2 |
| GSM359896 |
U0126-treated chondrocytes, biological replicate 1 |
| GSM359897 |
U0126-treated chondrocytes, biological replicate 2 |
| GSM359898 |
TNF-a treated chondrocytes, biological replicate 1 |
| GSM359899 |
TNF-a treated chondrocytes, biological replicate 2 |
| GSM359900 |
U0126 and TNF-a-treated chondrocytes, biological replicate 1 |
| GSM359901 |
U0126 and TNF-a-treated chondrocytes, biological replicate 2 |
|
| Relations |
| BioProject |
PRJNA111789 |
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