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| Status |
Public on Jun 30, 2020 |
| Title |
Genome-wide maps of RNA polymerase II in WT and ssu72 mutant cells [S5_S7_ChIPseq] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Deletion of ssu72 gene leads to a transcription elongation defect and the increase in the level of Ser5 and Ser7 phosphorylation in carboxy-terminal domain (CTD) of Rpb1
Phosphorylation of the RNA polymerase II C-terminal domain on heptad Y1S2P3T4S5P6S7 coordinates key events during transcription and when its deregulation leads to defects in transcription and RNA processing. Here we report that histone deacetylase complex activity of the fission yeast Hos2/Set3 complex plays an important role in suppressing cryptic initiation of the antisense transcription when the C-terminal domain phosphorylation is dysregulated due to the loss of Ssu72 phosphatase. Interestingly, while single hos2/set3 mutants have little effect, loss of hos2 or set3 in addition to ssu72? leads to synergistic increase in antisense transcription globally and correlates with increases genomic instability and formation of deleterious R-loop structures across these regions. We propose that Ssu72 is essential to suppress initiation of cryptic transcription in the 3?end of the RNA polymerase II transcribed regions. In the absence of Ssu72, antisense transcription is supressed by the Hos2/Set3 dependent surveillance mechanism to maintain genome stability.
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| |
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| Overall design |
Ser5-P CTD, and Ser7-P CTD ChIP-seq in WT and ssu72Δ
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| |
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| Contributor(s) |
Heo D, Kuś K, Kecman T, Tan-Wong SM, Zenkin N, Proudfoot NJ, Vasiljeva L |
| Citation(s) |
34496258 |
| Submission date |
Jan 31, 2020 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Dong-hyuk Heo |
| E-mail(s) |
heo.donghyuk@gmail.com
|
| Organization name |
University of Oxford
|
| Department |
Department of Biochemistry
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3QU |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL20584 |
Illumina NextSeq 500 (Schizosaccharomyces pombe) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE144603 |
Ssu72 is essential to suppress initiation of cryptic transcription in the 3' end of the RNA polymerase II transcribed regions. |
|
| Relations |
| BioProject |
PRJNA604123 |
| SRA |
SRP246316 |
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