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| Status |
Public on Apr 19, 2021 |
| Title |
Phase separation drives aberrant chromatin looping and cancer development [RNA-Seq] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Development of cancer is intimately associated with genetic abnormalities that target proteins with intrinsically disordered regions (IDRs). In human hematological malignancies, recurrent chromosomal translocation of nucleoporin (NUP98 or NUP214) generates an aberrant chimera that invariably retains nucleoporin?s IDR, tandemly dispersed phenylalanine-and-glycine (FG) repeats. However, it remains elusive how unstructured IDRs contribute to oncogenesis. We show that IDR harbored within NUP98-HOXA9, a homeodomain-containing transcription factor (TF) chimera recurrently detected in leukemias, is essential for establishing liquid-liquid phase separation (LLPS) puncta of chimera and for inducing leukemic transformation. Strikingly, LLPS of NUP98-HOXA9 not only promotes chromatin occupancy of chimera TFs but is also required for formation of a broad, ?super-enhancer?-like binding pattern, typically seen at a battery of leukemogenic genes, potentiating their transcriptional activation. Artificial HOX chimera (FUS-HOXA9), created by replacing NUP98?s FG repeats with an unrelated LLPS-forming IDR of FUS, had similar enhancement effects on chimera?s genome-wide binding and target gene activation. Hi-C mapping further demonstrated that phase-separated NUP98-HOXA9 induces CTCF-independent chromatin looping enriched at proto-oncogenes. Together, this report describes a proof-of-principle example wherein cancer acquires mutation to establish oncogenic TF condensates via phase separation, which simultaneously enhances their genomic targeting and induces organization of aberrant three-dimensional chromatin structure during tumorous transformation. As LLPS-competent molecules are frequently implicated in diseases, this mechanism can potentially be generalized to many malignant and pathological settings.
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| Overall design |
Differential expression analysis of transcriptome profiles in HEK293 or primary murine hematopoietic stem/progenitor cells (HSPCs) with stable expression of empty vector, wildtype NUP98-HOXA9, FS mutant NUP98-HOXA9, wildtype FUS-HOXA9 and YS mutant FUS-HOXA9.
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| Contributor(s) |
Ahn JH, Wang G, Uryu H |
| Citation(s) |
34163069 |
| Submission date |
Feb 01, 2020 |
| Last update date |
Jul 19, 2021 |
| Contact name |
Gang Greg Wang |
| E-mail(s) |
greg_wang@med.unc.edu
|
| Phone |
919-9665952
|
| Organization name |
UNC Lineberger Comprehensive Cancer Center
|
| Department |
Dept of Biochemistry and Biophysics
|
| Lab |
gregwanglab
|
| Street address |
450 West Drive
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7295 |
| Country |
USA |
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| Platforms (2) |
| GPL21626 |
NextSeq 550 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE144643 |
A phase separation mechanism underlies development of cancer and aberrant organization of three-dimensional chromatin structure |
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| Relations |
| BioProject |
PRJNA604380 |
| SRA |
SRP246521 |
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