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| Status |
Public on Dec 31, 2020 |
| Title |
Identification of new inhibitory molecules in T lymphocytes upon HCC development for new therapeutic targets |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Aim of the present study is the identification of new inhibitory targets on T lymphocytes which appear upon hepatocellular carcinoma (HCC) development and lead to a dysfunction of T cells by HCC microenvironment.
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| Overall design |
Hepatocellular carcinoma was induced by intrahepatic administration of two oncogenes (c-Myc and NrasG12V, abbreviation in the list below CN). Two control groups were injected with a single oncogene each (control group C and control group N), that did not result in cancer development. CD4 and CD8 cells were isolated from 6 tumor-bearing (TB) mice and from two groups (each with 6 mice) of healthy tumor-free (TF) control mice. In particular, CD4 and CD8 T cells were isolated from liver, liver-draining lymph nodes (relLN), not liver-draining lymph nodes (irrLN) and spleen. Microarray was performed on CD4 and CD8 T cells cells. In total, 3 groups by first microarray replicate were created with 4 organs each and in total 24 samples (CD4 and CD8). For the second replicate new 6 tumor-bearing and 6+6 tumor-free animals were used. In total, two replicates of microarray have been performed and in total 48 samples were analysed.
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| Contributor(s) |
Yevsa T, Hochnadel I, Geffers R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 05, 2020 |
| Last update date |
Jan 02, 2021 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
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| Phone |
+49 531-6181-3058
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| Organization name |
HCI - Helmholtz Centre for Infection Research
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| Department |
Dep. Molecular Bacteriology
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| Lab |
Genome Analytics
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| Street address |
Inhoffenstr. 7
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| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA604991 |
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