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| Status |
Public on Sep 23, 2020 |
| Title |
p63 Maintains the Proliferative Potential of Salivary Gland Stem/Progenitor Cells by Regulating Follistatin, a Key Mediator of TGF-b/Activin Signaling [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Np63+ve cells are multipotent and maintain all epithelial cell lineages of the embryonic and adult salivary gland (SG). However, the molecular mechanisms by which Np63 regulates stem/progenitor (SP) cell populations in the SG remains elusive. To better understand Np63 s role in directing cell fate choices, here we have utilized Np63-null adult mice and primary salivary cell cultures to probe alterations in SP cell differentiation and function. Specifically, we have generated bulk RNA-seq and scRNA-seq data from Np63-null adult mice and p63 and H3K27Ac ChIP-seq data from primary salivary cell cultures. These genomic and epigenomic data sets were leveraged to interrogate altered SG cellular identities and differentiation states resulting from the loss of Np63. Our studies reveal that ablation of Np63 results in a loss of the SP cell population and skewed SG differentiation that is modulated by dysregulated TGF- /Activin signaling. Our findings offer new molecular revelations into the SP cell gene regulatory networks that are likely to be relevant for normal or diseased SG states.
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| Overall design |
Analysis of gene expression changes in control and p63KO salivary glands using high throughput sequencing
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| Contributor(s) |
Min S, Oyelakin A, Gluck C, Bard J, Song C, Kirsten S, Che M, Flores E, Sinha S, Romano R |
| Citation(s) |
32932139 |
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https://doi.org/10.1016/j.isci.2020.101524
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| Submission date |
Feb 13, 2020 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Jonathan E Bard |
| E-mail(s) |
jbard@buffalo.edu
|
| Organization name |
University at Buffalo
|
| Department |
Genomics and Bioinformatics Core
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| Street address |
701 Ellicott Street
|
| City |
Buffalo |
| State/province |
New York |
| ZIP/Postal code |
14203 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE145268 |
p63 Maintains the Proliferative Potential of Salivary Gland Stem/Progenitor Cells by Regulating Follistatin, a Key Mediator of TGF-b/Activin Signaling |
|
| Relations |
| BioProject |
PRJNA606573 |
| SRA |
SRP249338 |
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