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| Status |
Public on Jan 24, 2009 |
| Title |
Expression profiling of HNF4-dependent transcripts in Drosophila larvae |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
We show that dHNF4 null mutant larvae are sensitive to starvation. Starved mutant larvae consume glycogen normally, but retain lipids in their midgut and fat body, and have increased levels of long chain fatty acids, suggesting that they are unable to efficiently mobilize stored fat for energy. HNF4 dependent transcripts were identified under fed and starved conditions. Microarray studies indicate reduced expression of genes that control lipid catabolism and beta-oxidation. Late second instar control and HNF4 mutant larvae were collected and fed or starved for 24 hours, and then RNA was isolated for analysis. Taken together, our results support a feed-forward model for dHNF4, in which fatty acids released from triglycerides activate the receptor, inducing enzymes that drive fatty acid oxidation for energy production.
Keywords: expression profiling, HNF4, mutants, Affymetrix, Drosophila 2.0, starvation, TAG, fatty acid, beta-oxidation
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| Overall design |
Staged late second instar control larvae that were transheterozygous for precise excisions of the EP2449 and KG08976 P-elements, and dHNF4D33/dHNF4D17 mutant larvae, were either fed or starved for 24 hrs in a 55mm petri dish containing Whatman paper soaked with PBS. RNA was extracted from the apparently healthy animals that molted to the third instar using Trizol (Gibco) and purified on RNAeasy columns (Qiagen). All samples were prepared in triplicate to facilitate subsequent statistical analysis. Probe labeling, hybridization to Affymetrix GeneChip® Drosophila Genome 2.0 Arrays.
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| Contributor(s) |
Thummel CS, Palanker L, Lam G, Tennessen J |
| Citation(s) |
19254568 |
| Submission date |
Jan 23, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Carl Thummel |
| Organization name |
University of Utah
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| Department |
Human Genetics
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| Lab |
Carl Thummel
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| Street address |
15 N 2030 E Rm 2100
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| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112-5330 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (12)
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| GSM363632 |
precise excision control fed L3 replicate 1 |
| GSM363633 |
precise excision control fed L3 replicate 2 |
| GSM363634 |
precise excision control fed L3 replicate 3 |
| GSM363635 |
HNF4 null fed L3 replicate 1 |
| GSM363636 |
HNF4 null fed L3 replicate 2 |
| GSM363637 |
HNF4 null fed L3 replicate 3 |
| GSM363638 |
precise excision control starved L3 replicate 1 |
| GSM363639 |
precise excision control starved L3 replicate 2 |
| GSM363640 |
precise excision control starved L3 replicate 3 |
| GSM363641 |
HNF4 null starved L3 replicate 1 |
| GSM363642 |
HNF4 null starved L3 replicate 2 |
| GSM363643 |
HNF4 null starved L3 replicate 3 |
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| Relations |
| BioProject |
PRJNA111609 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14531_RAW.tar |
26.3 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE14531_dCHIP_norm_data.txt |
930.9 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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