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| Status |
Public on Jan 01, 2021 |
| Title |
Coordinated Regulation of Smooth Muscle Cell Remodeling by Non-coding and Coding products of Phenotype Switching Regulator Gene. |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We identified a novel nuclear peptide Arteridin encoded by an annotated lncRNA Phenotype Swithching Regulator (PSR). To test the DNA binding capacity of Arteridin, we perfomed Arteridin ChIP-seq. We found both Arteridin and lncPSR transcript could induce vascular smooth muscle cell phenothype switching. We also found the interaction between Arteridin, lncPSR and transcription factor YBX1. To further illustrate how Arteridin and lncPSR work coordinately, we performed YBX1 ChIP-seq in VSMCs with Arteridin-FLAG overexpression or lncPSR ATT mutant overexpression.
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| Overall design |
Arteridin ChIP-seq was performed in VSMC A10 cells with or without Arteridin overexpression. YBX1 ChIP-seq was performed in VSMC A10 cells without treatment, with Arteridin-FLAG overexpression , and with lncPSR ATT mutant overexpression.
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| Contributor(s) |
Yu J, Zhang B |
| Citation missing |
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| Submission date |
Feb 26, 2020 |
| Last update date |
Jan 04, 2021 |
| Contact name |
Junyi Yu |
| E-mail(s) |
junyiyuamu@gmail.com
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| Phone |
(+86)13032388928
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| Organization name |
Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.
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| Department |
Department of Cardiology
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| Lab |
Chongqing Institute of Cardiology
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| Street address |
10th Changjiangzhilu Road, Yuzhong District
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| City |
Chongqing |
| ZIP/Postal code |
400042 |
| Country |
China |
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| Platforms (2) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
| GPL24688 |
HiSeq X Ten (Rattus norvegicus) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA608893 |
| SRA |
SRP250806 |
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