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| Status |
Public on Apr 13, 2020 |
| Title |
The Loss of TBK1 Kinase Activity in Motor Neurons or in All Cell Types Differentially Impacts ALS Disease Progression in SOD1 Mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
DNA sequence variants in the TBK1 gene associate with or cause sporadic or familial amyotrophic lateral sclerosis (ALS). Here we show that mice bearing human ALS-associated TBK1 missense loss-of-function mutations, or mice in which the Tbk1 gene is selectively deleted in motor neurons, do not display
a neurodegenerative disease phenotype. However, loss of TBK1 function in motor neurons of the SOD1G93A mouse model of ALS impairs autophagy, increases SOD1 aggregation, and accelerates early disease onset without affecting lifespan. By contrast, point mutations that decrease TBK1 kinase activity in all cells also accelerate disease onset but extend the lifespan of SOD1 mice. This difference correlates with the failure to activate high levels of expression of interferon-inducible genes in glia. We conclude that loss of TBK1 kinase activity impacts ALS disease progression through distinct pathways in different spinal cord cell types and further implicate the importance of glia in neurodegeneration.
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| Overall design |
Dissection of pathways affected by TBK1 loss of function mutations in the ALS spinal cord
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| Contributor(s) |
Gerbino V, Maniatis T |
| Citation(s) |
32220666 |
| Submission date |
Mar 01, 2020 |
| Last update date |
Apr 15, 2020 |
| Contact name |
Valeria Gerbino |
| E-mail(s) |
vg2362@columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Zuckerman Mind Brain Behavior Institute
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| Lab |
Maniatis Lab
|
| Street address |
3227 Broadway
|
| City |
New york |
| State/province |
Ny |
| ZIP/Postal code |
10027 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA609549 |
| SRA |
SRP251172 |
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