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| Status |
Public on Nov 13, 2020 |
| Title |
Plasma extracellular vesicle miRNAs as potential biomarkers for superstimulatory response in cattle |
| Organism |
Bos taurus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The objective of this study was to analyze the extracellular vesicle microRNA (EV-miRNA) expression profiles in the blood plasma of heifers with variable response to superstimulation.
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| Overall design |
Oestrous cycles of crossbred beef heifers were synchronized. On Day 10 after standing oestrus (=Day 0), superstimulation was induced by the administration of decreasing doses of follicle stimulating hormone twice a day for 4 d with PG administered with the 6th FSH injection followed by artificial insemination. Blood samples were collected on Day 7 of the unstimulated (U) and superstimulated (S) cycle from each heifer. All heifers were slaughtered on Day 7 of the S cycle. A subset of high (H, n=3) and low (L, n=3) responder heifers was selected depending on the ovarian response to superstimulation. Small RNA libraries were constructed and miRNA profiles were sequenced.
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| Contributor(s) |
Gad A, Sánchez JM, Browne JA, Laurincik J, Prochazka R, Lonergan P |
| Citation(s) |
33154526 |
| Submission date |
Apr 07, 2020 |
| Last update date |
Nov 14, 2020 |
| Contact name |
Ahmed Gad |
| E-mail(s) |
ahmed.y.gad@agr.cu.edu.eg
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| Organization name |
Colorado State University
|
| Department |
Biomedical Sciences
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| Lab |
ARBL
|
| Street address |
3107 Rampart Rd
|
| City |
Fort Collins |
| State/province |
Colorado |
| ZIP/Postal code |
80521 |
| Country |
USA |
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| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA623536 |
| SRA |
SRP255589 |
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