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| Status |
Public on May 19, 2023 |
| Title |
Sox9 Deletion Causes Severe Intervertebral Disc Degeneration Characterized by Apoptosis, Matrix Remodeling, and Compartment-Specific Transcriptomic Changes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptomic analysis of nucleus pulposus (NP) and annulus fibrosus (AF) tissues from intervertebral discs of 3-month-old mice with the conditional postnatal deletion of Sox9.
The transcription factor Sox9 is essential for the maintenance and health of the intervertebral disc of mice. We examined the transcriptomic profiles of nucleus pulposus and annulus fibrosus cells in control and Sox9 conditional knock-out mice using microarrays.
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| Overall design |
Tissues from the nucleus pulposus (NP) and annulus fibrosus (AF) were harvested from the intervertebral discs of four Sox9 control (Sox9CTR) (Sox9fl/fl) and four Sox9 conditional knock-out mice (Sox9cKO) (AcanCreERT2Sox9fl/fl) between 3 and 4 months of age. NP and AF tissues were separately pooled from lumbar and caudal discs in each animal for RNA extraction and hybridization using Affymetrix microarrays.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/33027692/
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| Contributor(s) |
Tsingas M, Ottone OK, Risbud MV |
| Citation(s) |
33027692 |
| Submission date |
May 19, 2020 |
| Last update date |
Aug 20, 2023 |
| Contact name |
Makarand Risbud |
| E-mail(s) |
makarand.risbud@jefferson.edu
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| Organization name |
Thomas Jefferson University
|
| Street address |
1015 Walnut Street
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA633860 |
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