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Series GSE151306 Query DataSets for GSE151306
Status Public on Jul 30, 2020
Title iPS-derived early oligodendrocyte progenitor cells from SPMS patients reveal deficient in vitro cell migration stimulation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Secondary progressive multiple sclerosis (SPMS) most challenging aspect is the lack of efficient regenerative response for remyelination, which is carried out by the endogenous population of adult oligoprogenitor cells (OPCs) after proper activation. OPCs must proliferate and migrate to the lesion and then differentiate into mature oligodendrocytes. To investigate the OPC cellular component in SPMS we developed iPSCs from SPMS affected donors and age matched controls (CT). We confirmed their efficient and similar OPC differentiation capacity, although we reported SPMS-OPCs are transcriptional distinguishable from their CT counterparts. Analysis of OPC-generated conditioned media (CM) also evidenced differences in protein secretion. We further confirmed SPMS-OPC CM presents a deficient capacity to stimulate OPC in vitro migration that can be compensated by exogenous addition of specific components. Our results provide an SPMS-OPC cellular model and encouraging venues to study potential cell communication deficiencies in the progressive form of MS for future treatment strategies.
 
Overall design Four biological replicates from SPMS adult donors (01-04) and three biological replicates from adult healthy donors (01-03) were used for analysis, iPSC reprogramming and OPC differentiation.
 
Contributor(s) Lopez-Caraballo L, Martorell-Marugán J, Carmona-Sáez P, Gonzalez-Munoz E
Citation(s) 32751289
Submission date May 27, 2020
Last update date Sep 08, 2020
Contact name Jordi Martorell-Marugan
Organization name GENyO
Department Bioinformatics Unit
Street address Avenida de la Ilustración 114
City Granada
ZIP/Postal code 18016
Country Spain
 
Platforms (2)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
GPL23159 [Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay)
Samples (23)
GSM4572201 primary MnSCs from healthy donor-_biological rep1
GSM4572202 primary MnSCs from healthy donor-_biological rep2
GSM4572203 primary MnSCs from healthy donor-_biological rep3
Relations
BioProject PRJNA635419

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151306_Illumina_non-normalized.txt.gz 3.1 Mb (ftp)(http) TXT
GSE151306_Illumina_processed.txt.gz 2.9 Mb (ftp)(http) TXT
GSE151306_RAW.tar 43.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record
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