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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 16, 2021 |
| Title |
Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Primordial germ cells (PGCs) are the founding population of the germ cell lineage that undergo a multistep process to generate spermatozoa or oocytes. Establishing an appropriate culture system for PGCs is a key challenge in reproductive biology. By a chemical screening using mouse PGC-like cells (mPGCLCs), which were induced from mouse embryonic stem cells, we reported previously that forskolin and rolipram synergistically enhanced the proliferation/survival of mPGCLCs with an average expansion rate of ∼20-fold. In the present study, we evaluated other chemicals or cytokines to see whether they would improve the current mPGCLC culture system. Among the chemicals and cytokines examined, in the presence of forskolin and rolipram, cyclosporin A (CsA) and fibroblast growth factors (FGFs: FGF2 and FGF10) effectively enhanced the expansion of mPGCLCs in vitro (∼50-fold on average). During the expansion by CsA or FGFs, mPGCLCs comprehensively erased their DNA methylation to acquire a profile equivalent to that of gonadal germ cells in vivo, while maintaining their highly motile phenotype as well as their transcriptional properties as sexually uncommitted PGCs. Importantly, these mPGCLCs robustly contributed to spermatogenesis and produced fertile offspring. Furthermore, mouse PGCs (mPGCs) cultured with CsA ex vivo showed transcriptomes and DNA methylomes similar to those of cultured mPGCLCs. The improved culture system for mPGCLCs/mPGCs would be instructive for addressing key questions in PGC biology, including the mechanisms for germ cell migration, epigenetic reprogramming, and sex determination of the germline.
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| Overall design |
Transcriptome analysis of mouse PGCLC culture suppoted by Cyclosporin A and FGF signaling.
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| Contributor(s) |
Ohta H, Yabuta Y, Kurimoto K, Nakamura T, Murase Y, Yamamoto T, Saitou M |
| Citation(s) |
33079185 |
| Submission date |
May 29, 2020 |
| Last update date |
May 18, 2021 |
| Contact name |
Yukihiro Yabuta |
| E-mail(s) |
yabyab@anat2.med.kyoto-u.ac.jp
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| Organization name |
Kyoto University, Graduate school of medicine
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| Department |
Anatomy and Cell Biology
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| Street address |
Yoshida-Konoe-cho, Sakyo-ku
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| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (18)
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| GSM4578123 |
EpiLC, rep2 |
| GSM4578124 |
d4 mPGCLC, rep1 |
| GSM4578125 |
d4 mPGCLC, rep2 |
| GSM4578126 |
d4c7+FR10, rep1 |
| GSM4578127 |
d4c7+FR10, rep2 |
| GSM4578128 |
d4c7+CsA, rep1 |
| GSM4578129 |
d4c7+CsA, rep2 |
| GSM4578130 |
d4c7+FGF10, rep1 |
| GSM4578131 |
d4c7+FGF10, rep2 |
| GSM4578132 |
d4c7+FGF2, rep1 |
| GSM4578133 |
d4c7+FGF2, rep2 |
| GSM4578134 |
E9.5c5 FR10, rep1 |
| GSM4578135 |
E9.5c5 FR10, rep2 |
| GSM4578136 |
E9.5c5+CsA, rep1 |
| GSM4578137 |
E9.5c5+CsA, rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE151446 |
Cyclosporin A and FGF signaling support the proliferation/survival of mouse primordial germ cell-like cells in vitro. |
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| Relations |
| BioProject |
PRJNA635877 |
| SRA |
SRP265264 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE151444_RAW.tar |
5.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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