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Status |
Public on Dec 18, 2020 |
Title |
Ribosome-bound Get4/5 facilitate the capture of tail anchored proteins by Sgt2 in yeast |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Other
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Summary |
The guided entry of tail-anchored proteins (GET) pathway assists in the proper delivery of tail-anchored (TA) proteins to the ER. Here we uncover how the yeast GET pathway components Get4/5 mediate capture of TA proteins by Sgt2, which interacts with TA sequences and hands them over to the targeting component Get3. Get4/5 binds directly and with high affinity to ribosomes, positions Sgt2 close to the ribosomal tunnel exit, and facilitates the capture of TA proteins by Sgt2. The contact sites of Get4/5 on the ribosome overlap with those of SRP, the factor mediating cotranslational ER-targeting of proteins containing internal TM domains. Exposure of nascent, internal TM domains at the tunnel exit induces high-affinity ribosome-binding of SRP, which in turn prevents ribosome-binding of Get4/5. In this way, the position of TM domains within nascent ER-targeted proteins mediates partitioning into either the GET or SRP pathway directly at the ribosomal tunnel exit.
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Overall design |
Wild-type BY4741 yeast cells or cells expressing genomically C-terminally His-Tev-ProtA-tagged Get4 were analysed by CRAC (Bohnsack et al., 2012).
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Contributor(s) |
Zhang Y, de Laurentiis E, Bohnsack KE, Wahlig M, Ranjan N, Gruseck S, Hackert P, Wölfle T, Rodnina M, Schwappach B, Rospert S |
Citation(s) |
33542241 |
Submission date |
Jun 02, 2020 |
Last update date |
Feb 22, 2021 |
Contact name |
Katherine Bohnsack |
E-mail(s) |
katherine.bohnsack@med.uni-goettingen.de
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Organization name |
University Medical Centre Göttingen
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Street address |
Humboldtallee 23
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City |
Göttingen |
ZIP/Postal code |
37073 |
Country |
Germany |
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Platforms (1) |
GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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Samples (3) |
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Relations |
BioProject |
PRJNA636793 |
SRA |
SRP265643 |
Supplementary file |
Size |
Download |
File type/resource |
GSE151664_RAW.tar |
100.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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