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| Status |
Public on Mar 09, 2010 |
| Title |
Expression profiling of nasopharyngeal carcinoma cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify the deregulated genes which may involved in the carcinogenesis of nasopharyngeal carcinoma (NPC).
To identify potential tumor suppressor genes / oncogenes of NPC, we have employed whole genome microarray expression profiling as a discovery platform to analyze two NPC cell lines, TW01 and HONE1. For the purpose to filter out differential genes may not related to carcinogenesis of NPC, a gene expression database for premalignant nasopharyngeal epithelial cells was obtained from NP460hTert cells, and we used the commercial universal human RNA (stratagene) as common reference for all microarray experiments. A total of 1272 genes were commonly up-regulated (fold change > 2) and 1810 genes were commonly down-regulated (fold change < 0.5) in TW01 and HONE1 cell lines, whereas not deregulated in NP460hTert cells.
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| Overall design |
Four samples including two NPC cell lines (TW01 and HONE1), an immortalized premalignant epithelial cell line NP460hTert, and the commercial universal human RNA (common reference) were analyzed. Differential genes found in NP460hTert were not considered as NPC-related. Biological replicate experiments were performed for each sample.
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| Contributor(s) |
Lee CH, Chen CJ |
| Citation(s) |
19336547 |
| Submission date |
Mar 10, 2009 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Chia Huei Lee |
| E-mail(s) |
chlee124@nhri.edu.tw
|
| Organization name |
Taiwan National Health Research Institute
|
| Department |
National Institute of Cancer Research
|
| Street address |
35, Keyan Rd.
|
| City |
Zhunan Miaoli County |
| ZIP/Postal code |
114 |
| Country |
Taiwan |
| |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE15191 |
Nasopharyngeal carcinoma cell lines TW01 and HONE1: transcriptomic and genomic analyses |
|
| Relations |
| BioProject |
PRJNA123123 |
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