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| Status |
Public on Dec 15, 2020 |
| Title |
Gene expression data in A172 cell line transfected with Anti-miR-196a-5p miRNA or its respective negative control under hypoxia (0.2% O2) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
miR-196a-5p is robustly upregulated under hypoxia in GBM cell lines and is highly expressed in GBM tumour samples. This study identifies the genes differentially expressed upon miR-196a-5p inhibition in A172 glioblastoma cell line under hypoxia (0.2% O2).
GeneChip PrimeView Human Gene Expression Array was used to assess mRNA expression profile in response to miR-196a-5p inhibition in A172 cell line under hypoxia (0.2% O2).
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| Overall design |
A172 cells were transfected with either Anti-miR-196a-5p miRNA or its respective negative control under hypoxia (0.2% O2). The cells were harvested in hypoxia workstation for RNA isolation 48 hours post-transfection. RNA was used for the gene expression analysis using Affymetrix GeneChip PrimeView system.
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| Contributor(s) |
Takkar S, Kulshreshtha R, Ghosh S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 16, 2020 |
| Last update date |
Dec 18, 2020 |
| Contact name |
Dr. Ritu Kulshreshtha |
| E-mail(s) |
ritu@dbeb.iitd.ac.in
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| Phone |
011-26591017
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| Organization name |
Indian Institute of Technology
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| Department |
Dept of Biochemical Engineering and Biotechnology
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| Lab |
Biochemical Engineering and Biotechnolog
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| Street address |
Room 134, Block I
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| City |
New Delhi |
| State/province |
New Delhi |
| ZIP/Postal code |
110016 |
| Country |
India |
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| Platforms (1) |
| GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA639773 |
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